Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I3 R2
|
52 |
92.4 |
4932871 |
87.1% |
4296530 |
63.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,072,048 |
A→T |
intergenic (‑88/‑131) |
ygjH ← / → ebgR |
conserved hypothetical protein/DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,072,048 | 0 | A | T | 100.0%
| 125.7
/ NA
| 40 | intergenic (‑88/‑131) | ygjH/ebgR | conserved hypothetical protein/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (18/22); total (18/22) |
ACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAG > minE/2071987‑2072115
|
aCTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTc < 1:3552605/70‑1 (MQ=255)
aCTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTc < 1:2491014/70‑1 (MQ=255)
cTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTc < 1:2496575/69‑1 (MQ=255)
aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg < 1:1300930/69‑1 (MQ=255)
aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg < 1:4307412/69‑1 (MQ=255)
aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg < 1:3244019/69‑1 (MQ=255)
aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg < 1:2324012/69‑1 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:935919/1‑71 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:64614/1‑71 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:4559310/1‑71 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:3893052/1‑71 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:3127841/1‑71 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:1866477/1‑71 (MQ=255)
gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTAGTGAGCCGCtg > 1:3369998/1‑70 (MQ=255)
aaaGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGt < 1:1080054/58‑1 (MQ=255)
aGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg < 1:2038042/57‑1 (MQ=255)
aaTCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACa < 1:2600359/68‑1 (MQ=255)
ccTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc > 1:2458464/1‑70 (MQ=255)
ccTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc > 1:1326891/1‑70 (MQ=255)
ccTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc > 1:588721/1‑70 (MQ=255)
gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc > 1:1575414/1‑67 (MQ=255)
gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:1562307/1‑68 (MQ=255)
gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:1418133/1‑68 (MQ=255)
gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:539397/1‑68 (MQ=255)
gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:2792233/1‑68 (MQ=255)
gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:2069703/1‑68 (MQ=255)
gTAGCCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:3211696/1‑68 (MQ=255)
tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:705172/71‑1 (MQ=255)
tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:761056/71‑1 (MQ=255)
tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:3654755/71‑1 (MQ=255)
tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:3952181/71‑1 (MQ=255)
tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:2699424/71‑1 (MQ=255)
ggCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACa < 1:4101120/59‑1 (MQ=255)
tAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAAcgc > 1:3046812/1‑71 (MQ=255)
gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt < 1:1602914/71‑1 (MQ=255)
gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt < 1:4570650/71‑1 (MQ=255)
gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt < 1:260189/71‑1 (MQ=255)
gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt < 1:749385/71‑1 (MQ=255)
gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt < 1:249566/71‑1 (MQ=255)
agaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttattt < 1:4557772/71‑1 (MQ=255)
gtgtTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg < 1:4578698/71‑1 (MQ=255)
gtgtTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg < 1:2989998/71‑1 (MQ=255)
gtgtTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg < 1:2386286/71‑1 (MQ=255)
|
ACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAG > minE/2071987‑2072115
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
