Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I3 R2	52	92.4	4932871	87.1%	4296530	63.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,073,779	G→A	G145D (GGT→GAT)	ebgA →	cryptic beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,073,779	0	G	A	100.0%	476.7 / NA	144	G145D (GGT→GAT)	ebgA	cryptic beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (65/79); total (65/79)

Only 100 of 144 total aligned reads displayed.

GCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAA  >  minE/2073714‑2073846

  aGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:2044563/69‑1 (MQ=255)

  aGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:2784481/69‑1 (MQ=255)

  aGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:2515706/69‑1 (MQ=255)

  aGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:513580/69‑1 (MQ=255)

  aGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:516992/69‑1 (MQ=255)

  aGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:4377938/69‑1 (MQ=255)

   gtgTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGt                                                                <  1:4776257/66‑1 (MQ=255)

      tAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTgg                                                          >  1:23593/1‑71 (MQ=255)

      tAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTgg                                                          >  1:2629372/1‑71 (MQ=255)

      tAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTgg                                                          >  1:1996808/1‑71 (MQ=255)

      tAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTgg                                                          >  1:2956387/1‑71 (MQ=255)

              cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAg                                                   >  1:1493876/1‑70 (MQ=255)

              cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAg                                                   >  1:2250076/1‑70 (MQ=255)

              cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAg                                                   >  1:4255640/1‑70 (MQ=255)

              cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAg                                                   >  1:3976762/1‑70 (MQ=255)

              cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAg                                                   >  1:910001/1‑70 (MQ=255)

              cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:486366/71‑1 (MQ=255)

               gCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:349339/70‑1 (MQ=255)

               gCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:2570932/70‑1 (MQ=255)

                   aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             >  1:3180326/1‑71 (MQ=255)

                   aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             >  1:2482552/1‑71 (MQ=255)

                   aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             >  1:3011226/1‑71 (MQ=255)

                   aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             <  1:3540082/71‑1 (MQ=255)

                   aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             <  1:2649018/71‑1 (MQ=255)

                   aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             >  1:1431544/1‑71 (MQ=255)

                    ttAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             <  1:1909473/70‑1 (MQ=255)

                    ttAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAggg                                             <  1:1772006/70‑1 (MQ=255)

                     tAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTa                                                               >  1:3367035/1‑51 (MQ=255)

                     tAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTa                                                               >  1:1864087/1‑51 (MQ=255)

                     tAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTa                                                               >  1:3545855/1‑51 (MQ=255)

                     tAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTa                                                               >  1:4416986/1‑51 (MQ=255)

                      aaaTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAg                                          >  1:3043076/1‑71 (MQ=255)

                        aTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:3227208/71‑1 (MQ=255)

                        aTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:4224459/71‑1 (MQ=255)

                         tttGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:4379010/70‑1 (MQ=255)

                             aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTg                                   <  1:2091082/71‑1 (MQ=255)

                             aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTg                                   <  1:4578780/71‑1 (MQ=255)

                             aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTg                                   <  1:4093570/71‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:3029974/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:3119040/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:3291880/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:3309510/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:1031445/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:3648726/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:2932635/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:409422/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:1205754/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:797458/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:1592543/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:4755666/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:2193606/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:4657680/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:2328250/41‑1 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCg                               >  1:4517785/1‑69 (MQ=255)

                                   tCGAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCg                               >  1:1450146/1‑69 (MQ=255)

                                    cGAAACCTATTTAGAAGTCTATGTTAACGATCAGTATGTGGGtt                                                       <  1:4701234/44‑1 (MQ=38)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCTGCa                                                 >  1:4041798/1‑49 (MQ=39)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCa                                                 >  1:2343051/1‑49 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCa                                                 >  1:2395755/1‑49 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCa                                                 >  1:4886028/1‑49 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCa                                                 >  1:4503241/1‑49 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCa                                                 >  1:4221556/1‑49 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCa                                                 >  1:4418884/1‑49 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                             >  1:2017553/1‑69 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                             >  1:4016641/1‑69 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                             >  1:1451565/1‑69 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                             >  1:2676605/1‑69 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                             >  1:4689655/1‑69 (MQ=255)

                                     gAAACCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCa                             >  1:2181153/1‑69 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:771382/47‑1 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:1064133/47‑1 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:1153266/47‑1 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:1791806/47‑1 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:2179095/47‑1 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCagag                          <  1:2606576/71‑1 (MQ=255)

                                      aaaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCagag                          <  1:4385312/71‑1 (MQ=255)

                                       aaCCTATTTTGAAGTCTATGTTAACGATCAGTAtgtg                                                           <  1:3791453/37‑1 (MQ=255)

                                       aaCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGc                                                  <  1:2858513/46‑1 (MQ=255)

                                        aCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCaa                                                >  1:2375602/1‑47 (MQ=255)

                                        aCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCaa                                                >  1:1428246/1‑47 (MQ=255)

                                        aCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCaa                                                >  1:528881/1‑47 (MQ=255)

                                        aCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCaa                                                >  1:3678359/1‑47 (MQ=255)

                                        aCCTATTTTGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCaa                                                >  1:2152328/1‑47 (MQ=255)

                                          cTATTTTGAAGTCTATGCTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGttt                       >  1:4633502/1‑70 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:95138/50‑1 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:4010784/50‑1 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:1227481/50‑1 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:1887950/50‑1 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTc                                        <  1:2417224/50‑1 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACa                   <  1:1925956/71‑1 (MQ=255)

                                             ttttGAAGTCTATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACa                   <  1:2246846/71‑1 (MQ=255)

                                                       tATGTTAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGAt         >  1:2283947/1‑71 (MQ=255)

                                                           ttAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGc                                      >  1:1935059/1‑38 (MQ=255)

                                                           ttAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGc                                      >  1:1974351/1‑38 (MQ=255)

                                                           ttAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGc                                      >  1:1560233/1‑38 (MQ=255)

                                                           ttAACGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGc                                      >  1:3500943/1‑38 (MQ=255)

                                                              aCGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTaaa  <  1:4259461/71‑1 (MQ=255)

                                                              aCGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTaaa  <  1:735603/71‑1 (MQ=255)

                                                              aCGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTaaa  <  1:793565/71‑1 (MQ=255)

                                                              aCGATCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTaaa  <  1:3096144/71‑1 (MQ=255)

GCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAA  >  minE/2073714‑2073846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A