Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I3 R2	52	92.4	4932871	87.1%	4296530	63.5

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,379,816	C→T	intergenic (‑58/+37)	ubiE ← / ← rmuC	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase/predicted recombination limiting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,379,816	0	C	T	100.0%	74.8 / NA	24	intergenic (‑58/+37)	ubiE/rmuC	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase/predicted recombination limiting protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/12); total (12/12)

CATCAAAAAATTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGCCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGACTGCTGATTATATTCAT  >  minE/2379772‑2379878

catcaAAAAATTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAactac                                      >  1:2984527/1‑71 (MQ=255)

    aaaaaaTTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTcc                                   >  1:616917/1‑70 (MQ=255)

    aaaaaaTTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTcc                                   >  1:3080452/1‑70 (MQ=255)

    aaaaaaTTGGTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTcc                                   >  1:4740125/1‑70 (MQ=255)

       aaaTTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTc                                    >  1:3738079/1‑66 (MQ=255)

       aaaTTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAAt                               >  1:1446956/1‑71 (MQ=255)

       aaaTTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAAt                               >  1:3735214/1‑71 (MQ=255)

               ccAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCGATGGGCTAg                        <  1:709948/70‑1 (MQ=255)

               ccAGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAg                        <  1:1891777/70‑1 (MQ=255)

               ccAGAAGTGTAACAGATAGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAg                        <  1:4019153/70‑1 (MQ=255)

                 aGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAgcgc                     <  1:239944/71‑1 (MQ=255)

                 aGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAgcgc                     <  1:219010/71‑1 (MQ=255)

                 aGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAgcgc                     <  1:571568/71‑1 (MQ=255)

                 aGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAgcgc                     <  1:2064635/71‑1 (MQ=255)

                 aGAAGTGTAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAgcgc                     <  1:1931532/71‑1 (MQ=255)

                        tAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGActgct              <  1:3015743/71‑1 (MQ=255)

                        tAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGActgct              <  1:3449953/71‑1 (MQ=255)

                        tAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGActgct              <  1:2520962/71‑1 (MQ=255)

                        tAACAGATTGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGActgct              <  1:4144635/71‑1 (MQ=255)

                                tGGGCGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGACTGCTGATTatat      >  1:427967/1‑71 (MQ=255)

                                tGGGAGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGACTGCTGATTatat      >  1:58821/1‑71 (MQ=255)

                                    cGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTGCCAATGGGCTAGCGCGACTGCTGATTATATtcat  >  1:3454655/1‑71 (MQ=255)

                                    cGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGACTGCTGATTATATtcat  >  1:3195739/1‑71 (MQ=255)

                                    cGTCGATGTCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGACTGCTGATTATATtcat  >  1:3332966/1‑71 (MQ=255)

CATCAAAAAATTGTTCCAGAAGTGTAACAGATTGGGCGTCGATGCCCTAGATTTCTACCCGGCTTAACTACTCCCAATGGGCTAGCGCGACTGCTGATTATATTCAT  >  minE/2379772‑2379878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A