Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I3 R2 52 92.4 4932871 87.1% 4296530 63.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,748,276 A→G S117G (AGC→GGC)  secY → preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,748,2760AG100.0% 415.2 / NA 110S117G (AGC→GGC) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (65/45);  total (65/45)

Only 100 of 110 total aligned reads displayed.

CTGACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGT  >  minE/2748210‑2748341
                                                                  |                                                                 
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:126769/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:61971/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:523627/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:78453/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3756390/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:1799852/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:4911113/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:190017/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:4184726/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:4224328/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3389495/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:2025188/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3173725/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:2552/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:1135605/1‑69 (MQ=255)
ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3265440/1‑69 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGt                                                             <  1:2662765/67‑1 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGt                                                             <  1:3886847/67‑1 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:722146/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:520466/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4445513/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:1026271/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:2343133/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:442935/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3386493/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4360636/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:798152/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3637031/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:1349568/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4931720/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:2932303/1‑68 (MQ=255)
      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4143940/1‑68 (MQ=255)
          ttCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:2136345/71‑1 (MQ=255)
           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3076567/1‑63 (MQ=255)
           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4531575/1‑63 (MQ=255)
           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:2630988/1‑63 (MQ=255)
           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3855675/1‑63 (MQ=255)
           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:141436/1‑63 (MQ=255)
           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     >  1:3653101/1‑70 (MQ=255)
             aCCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:2702766/65‑1 (MQ=255)
             aCCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:2199973/65‑1 (MQ=255)
               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:3284255/71‑1 (MQ=255)
               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:3808910/71‑1 (MQ=255)
               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:1055005/71‑1 (MQ=255)
               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:1811152/71‑1 (MQ=255)
               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:2929993/71‑1 (MQ=255)
               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:1254972/71‑1 (MQ=255)
                cAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                >  1:552150/1‑70 (MQ=255)
                       ggCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTg                                         <  1:1827429/70‑1 (MQ=255)
                        gCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGt                                       >  1:3133558/1‑71 (MQ=255)
                            aaaTTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:4542814/53‑1 (MQ=255)
                                 aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               >  1:3309463/1‑70 (MQ=255)
                                 aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               >  1:3621761/1‑70 (MQ=255)
                                 aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               >  1:4138696/1‑70 (MQ=255)
                                     aagaagGGGAGTCTGGTCGTCGTAAGATCGGCCAGt                                                             <  1:2696506/36‑1 (MQ=255)
                                     aagaagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:241978/49‑1 (MQ=255)
                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCa                        >  1:2041300/1‑70 (MQ=255)
                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCa                        >  1:1948171/1‑70 (MQ=255)
                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg                       >  1:117311/1‑71 (MQ=255)
                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg                       >  1:4492028/1‑71 (MQ=255)
                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg                       >  1:4067503/1‑71 (MQ=255)
                                         cggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:4400888/39‑1 (MQ=39)
                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:1294585/37‑1 (MQ=255)
                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:2552773/45‑1 (MQ=255)
                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:3921813/45‑1 (MQ=255)
                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:4041289/45‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:4695076/61‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1160180/61‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:3412085/61‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1737111/61‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:3358692/61‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:3037217/61‑1 (MQ=255)
                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1467079/61‑1 (MQ=255)
                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:2996784/35‑1 (MQ=255)
                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:4582731/38‑1 (MQ=255)
                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:165551/60‑1 (MQ=255)
                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:290056/60‑1 (MQ=255)
                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCg                    <  1:2802155/71‑1 (MQ=255)
                                               gTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1758562/56‑1 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:1557895/68‑1 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:4612469/68‑1 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:1149353/68‑1 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:336922/68‑1 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:451662/68‑1 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAtt           >  1:3245491/1‑71 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAtt           >  1:3803410/1‑71 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAtt           >  1:1174010/1‑71 (MQ=255)
                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAt            >  1:4597274/1‑70 (MQ=255)
                                                       gtcgtAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:1602888/65‑1 (MQ=255)
                                                          gtAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAcc     >  1:4544649/1‑71 (MQ=255)
                                                          gtAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAc      <  1:947591/70‑1 (MQ=255)
                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:3706075/1‑71 (MQ=255)
                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:3110816/1‑71 (MQ=255)
                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:2912482/1‑71 (MQ=255)
                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:1955178/1‑71 (MQ=255)
                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:1655907/1‑71 (MQ=255)
                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:157552/1‑71 (MQ=255)
                                                             aGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:2972308/1‑70 (MQ=255)
                                                             aGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGt  >  1:4579966/1‑71 (MQ=255)
                                                             aGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGt  >  1:3736232/1‑71 (MQ=255)
                                                                  |                                                                 
CTGACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGT  >  minE/2748210‑2748341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A