Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I2 R1
|
60 |
60.8 |
348784 |
8.9% |
31041 |
54.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,435,079 |
A→G |
R202R (CGT→CGC) |
purF ← |
amidophosphoribosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,435,079 | 0 | A | G | 100.0%
| 25.5
/ NA
| 15 | R202R (CGT→CGC) | purF | amidophosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/15); total (0/15) |
TATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGG > W3110S.gb/2435071‑2435117
|
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:1785/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:190575/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:194975/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:20198/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:228880/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:274461/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:301536/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:312186/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:6242/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:66848/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:79843/47‑1 (MQ=255)
tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:80832/47‑1 (MQ=255)
tatTCTGTGCGGGTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:226009/47‑1 (MQ=255)
atTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:252913/46‑1 (MQ=255)
atTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg < 1:316135/46‑1 (MQ=255)
|
TATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGG > W3110S.gb/2435071‑2435117
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
