Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I2 R1
|
60 |
60.8 |
348784 |
8.9% |
31041 |
54.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,538,196 |
G→C |
T114T (ACG→ACC) |
cysK → |
cysteine synthase A, O‑acetylserine sulfhydrolase A subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,538,196 | 0 | G | C | 99.7%
| 653.8
/ NA
| 312 | T114T (ACG→ACC) | cysK | cysteine synthase A, O‑acetylserine sulfhydrolase A subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (311/0); minor base A (1/0); total (312/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 312 total aligned reads displayed.
CCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGA > W3110S.gb/2538154‑2538198
|
ccgcAAGCTGCTGAAAGCGTTAGGTGCAACCCTGGTGCTGACCGa > 1:244117/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:285498/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:336113/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:331476/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:32748/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:314925/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:311150/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:308043/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:304943/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:301032/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:297396/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:2936/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:289524/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:287789/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:33939/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:284786/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:283602/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:280758/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:2779/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:276615/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:272379/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:27007/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:264938/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:261035/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:26000/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:257700/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:60527/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:97144/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:91777/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:88404/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:85872/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:83282/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:82569/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:78245/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:73460/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:70331/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:68655/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:649/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:64282/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:252903/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:58415/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:54022/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:50438/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:48492/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:4510/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:42359/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:38171/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:348048/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:34673/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:343420/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:341096/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:133196/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:17533/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:171838/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:168603/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:167050/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:162375/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:156662/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:150410/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:145752/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:144210/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:140954/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:137636/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:178812/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:128595/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:127623/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:125060/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:12159/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:119405/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:117213/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:114063/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:108630/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:107445/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:101908/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:101075/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:216181/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:251712/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:249193/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:247769/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:239292/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:237531/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:235256/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:232119/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:22561/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:222243/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:218132/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:217031/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:100678/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:212783/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:21054/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:206416/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:20238/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:2011/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:196239/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:195129/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:189788/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:187468/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:185070/1‑45 (MQ=255)
ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:183666/1‑45 (MQ=255)
ccgcAAGCTGATGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:131710/1‑45 (MQ=38)
|
CCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGA > W3110S.gb/2538154‑2538198
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
