Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F8 I0 R2	5	12.5	627370	96.8%	607294	61.4

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,892,019:1	+GC	100%	intergenic (‑437/+54)	gudP ← / ← ygdL	predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,892,019	1	.	G	100.0%	22.9 / NA	8	intergenic (‑437/+54)	gudP/ygdL	predicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (2/6); total (2/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*	minE	1,892,019	2	.	C	100.0%	23.2 / NA	8	intergenic (‑437/+54)	gudP/ygdL	predicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (2/6); total (2/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCG  >  minE/1891951‑1892062

||

cTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag                                              <  1:234570/70‑2 (MQ=255)

              aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                               >  1:28382/1‑71 (MQ=255)

                          tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                              <  1:209117/60‑1 (MQ=255)

                               aCCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt               <  1:34176/70‑1 (MQ=255)

                                 cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                   <  1:328245/48‑1 (MQ=255)

                                   cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg           >  1:44123/1‑70 (MQ=255)

                                            cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg  <  1:173470/70‑1 (MQ=255)

                                             aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                <  1:69556/39‑1 (MQ=37)

                                              tttACCGCTCGCTGAACATATCAGCTAAGCACAGa                                   <  1:313393/35‑1 (MQ=25)

||

CTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCG  >  minE/1891951‑1892062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A