Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F8 I0 R1
|
24 |
67.1 |
3369429 |
96.5% |
3251498 |
61.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,695,660 |
C→A |
20.1% |
R117L (CGC→CTC) |
yfhQ ← |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,695,660 | 0 | C | A | 20.1%
| 198.0
/ 19.6
| 64 | R117L (CGC→CTC) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (36/15); new base A (9/4); total (45/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGC > minE/1695590‑1695729
|
gTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAcgc > 1:373955/1‑71 (MQ=255)
ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:212631/1‑71 (MQ=255)
ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:593691/1‑71 (MQ=255)
ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:2717506/1‑71 (MQ=255)
ggTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGa > 1:1214368/1‑71 (MQ=255)
aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:482930/70‑1 (MQ=255)
aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:449104/70‑1 (MQ=255)
aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa > 1:1655328/1‑70 (MQ=255)
ccGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac < 1:3126556/71‑1 (MQ=255)
ccGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac < 1:2410907/71‑1 (MQ=255)
aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAacac > 1:1760758/1‑69 (MQ=255)
aaTAGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAac > 1:1656881/1‑67 (MQ=255)
aTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAg > 1:417782/1‑71 (MQ=255)
aTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAg < 1:1735357/71‑1 (MQ=255)
gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:744710/1‑57 (MQ=255)
gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:169989/1‑64 (MQ=255)
gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAaca > 1:1952189/1‑64 (MQ=255)
gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCaaa > 1:2050216/1‑62 (MQ=255)
aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:1400477/50‑1 (MQ=255)
aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:2903716/50‑1 (MQ=255)
aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:2276534/50‑1 (MQ=255)
aTGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTcgc < 1:2276275/50‑1 (MQ=255)
tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgg > 1:2781341/1‑71 (MQ=255)
tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgg > 1:535829/1‑71 (MQ=255)
agATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgg > 1:1567463/2‑71 (MQ=255)
gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCggg > 1:976124/1‑71 (MQ=255)
gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCggg > 1:1039168/1‑71 (MQ=255)
gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGa > 1:610229/1‑50 (MQ=255)
tAATGGAATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCgggg > 1:4483/1‑70 (MQ=255)
aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:727016/1‑51 (MQ=255)
aTGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:120518/1‑51 (MQ=255)
ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:210856/1‑49 (MQ=255)
ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:969232/1‑49 (MQ=255)
ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:1308527/1‑49 (MQ=255)
ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAttt > 1:1714670/1‑71 (MQ=255)
aTTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGcc > 1:2761636/1‑71 (MQ=255)
tttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTcgc < 1:1231023/38‑1 (MQ=255)
tttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTcgc < 1:487869/38‑1 (MQ=255)
tctcCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAACACCAg > 1:735747/1‑49 (MQ=38)
tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGa > 1:2170875/1‑38 (MQ=255)
tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAc > 1:2087690/1‑39 (MQ=255)
tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAc > 1:1088859/1‑39 (MQ=255)
tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCt > 1:782215/1‑71 (MQ=255)
tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCt > 1:3198238/1‑71 (MQ=255)
tGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:2618067/39‑1 (MQ=255)
tGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:2614086/39‑1 (MQ=255)
tGCAACTCTTCATTGGTCAAGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCtt > 1:815283/1‑70 (MQ=255)
aCTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGc < 1:10592/70‑1 (MQ=255)
ctctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGa > 1:713897/1‑71 (MQ=255)
tCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCt > 1:64808/1‑71 (MQ=255)
cATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGc < 1:1578125/69‑1 (MQ=255)
aTTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtt < 1:2135139/70‑1 (MQ=255)
ttGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt < 1:1202306/71‑1 (MQ=255)
ttGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt < 1:889647/71‑1 (MQ=255)
ttGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt < 1:1076948/71‑1 (MQ=255)
ggTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCtttt > 1:2341105/1‑69 (MQ=255)
tCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAgg > 1:211700/1‑71 (MQ=255)
tCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAgg > 1:2822608/1‑71 (MQ=255)
cAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGc > 1:3160091/1‑71 (MQ=255)
aaGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa > 1:811498/1‑54 (MQ=255)
aaGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa > 1:1001368/1‑54 (MQ=255)
aaGCCGACGAGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa > 1:2169880/1‑54 (MQ=255)
aGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCg > 1:300603/1‑71 (MQ=255)
gCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCg > 1:1566040/1‑70 (MQ=255)
ccGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCa > 1:525311/1‑71 (MQ=255)
gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:1986052/1‑71 (MQ=255)
gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:434225/1‑71 (MQ=255)
gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:3199907/1‑71 (MQ=255)
gcgcTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTcgc > 1:1334698/1‑71 (MQ=255)
|
GTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGC > minE/1695590‑1695729
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
