Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I3 R2 56 7.9 54550 14.3% 7800 51.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,178,858 A→G A266A (GCT→GCC)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,178,8580AG100.0% 102.0 / NA 51A266A (GCT→GCC) gatZD‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/0);  total (51/0)

ATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTG  >  W3110S.gb/2178824‑2178872
                                  |              
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTc    >  1:10696/3‑47 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCt   >  1:52875/3‑48 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:42976/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32049/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32084/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32364/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32437/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:36945/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:37587/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:37728/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:39050/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:40168/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:40788/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:42756/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:28812/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:42990/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:43643/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:43905/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:4407/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:46204/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:50103/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:50263/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:51902/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:53253/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:7966/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:8890/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:20440/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:10917/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:11505/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14038/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14405/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:1447/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14546/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14595/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:1608/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:16610/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:17766/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:18914/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:19272/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:29907/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:21325/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:21434/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:21582/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:22295/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:22695/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:24304/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:24726/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:24880/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:25241/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:26244/3‑49 (MQ=255)
aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:27919/3‑49 (MQ=255)
                                  |              
ATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTG  >  W3110S.gb/2178824‑2178872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A