Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I2 R2 38 6089.5 1044078 7.4% 77261 54.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,989,600 G→T intergenic (+35/+44) yecI → / ← yecJ predicted ferritin‑like protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,989,6000GT100.0% 900.9 / NA 369intergenic (+35/+44)yecI/yecJpredicted ferritin‑like protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (369/0);  total (369/0)

Only 100 of 369 total aligned reads displayed.

AATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTT  >  W3110S.gb/1989580‑1989641
                    |                                         
aaTGCCTTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:80873/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTg                           >  1:737449/1‑37 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTTATTTTTTTGAAGCAGCGGtt   >  1:347586/1‑61 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGaa            >  1:929665/1‑52 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGGAGCAGCGGttt  >  1:321471/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAag           >  1:80081/1‑53 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCgg     >  1:1034426/1‑59 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:730226/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:794177/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:784917/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:778047/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:775816/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:769724/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:755824/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:750735/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:747253/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:744587/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:591155/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:715863/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:700163/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:685220/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:677407/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:667034/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:649927/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:62721/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:61585/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:608645/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:60062/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:578492/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:891824/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:988179/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:979111/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:974455/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:96608/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:964088/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:94832/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:944097/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:922029/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:919812/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:896650/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:819221/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:883949/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:879459/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:872576/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:868386/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:857211/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:85301/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:849134/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:838534/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:830959/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:825771/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:186623/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:36024/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:357806/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:339952/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:314202/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:292539/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:283083/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:264556/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:248742/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:243534/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:229232/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:202237/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:37526/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:181216/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:174689/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:169794/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:16618/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:149810/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:137556/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:125687/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:108425/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1041706/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1027625/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1018317/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:462355/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1002147/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:56949/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:564591/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:549079/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:545073/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:532607/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:52182/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:507431/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:498820/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:490757/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:483004/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:586916/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:451292/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:445307/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:436177/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:430405/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:417739/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:41286/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:411983/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:398792/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:385557/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:377246/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGtt   >  1:697002/1‑61 (MQ=255)
aaTGCATAGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:277107/1‑62 (MQ=255)
                    |                                         
AATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTT  >  W3110S.gb/1989580‑1989641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A