Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I2 R2
|
42 |
122.2 |
1899532 |
52.7% |
1001053 |
56.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,427,572 |
A→C |
P303P (CCT→CCG) |
rffE ← |
UDP‑N‑acetyl glucosamine‑2‑epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,427,572 | 0 | A | C | 100.0%
| 33.1
/ NA
| 10 | P303P (CCT→CCG) | rffE | UDP‑N‑acetyl glucosamine‑2‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (0/10); total (0/10) |
GACGCTCAGTGGTATCGCGCATCACCAGCACAGGTTTCCCCAGCGAAGGCGC > minE/2427541‑2427592
|
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:1332451/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:236259/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:240261/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:279156/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:292840/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:317166/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:619459/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:775737/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:786082/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc < 1:916499/52‑1 (MQ=255)
|
GACGCTCAGTGGTATCGCGCATCACCAGCACAGGTTTCCCCAGCGAAGGCGC > minE/2427541‑2427592
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
