Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I2 R2 42 122.2 1899532 52.7% 1001053 56.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,694,258 C→T E124K (GAA→AAA)  yrfF ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,694,2580CT100.0% 438.4 / NA 130E124K (GAA→AAA) yrfFpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (130/0);  total (130/0)

Only 100 of 130 total aligned reads displayed.

CAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTA  >  minE/2694223‑2694284
                                   |                          
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa                     >  1:1662647/1‑43 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:1608498/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:1139462/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:861914/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:1103408/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:864738/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:1552372/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCt   >  1:686055/1‑61 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:460610/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:508306/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:512750/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:514807/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:517752/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:458221/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:440225/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:413320/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:344963/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:363552/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:35115/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:348851/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:582991/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:32581/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:306307/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:305280/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:276810/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:264914/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:25995/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:210962/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:190380/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:805948/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:98802/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:983098/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:9799/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:963860/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:93251/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:931392/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:897928/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:886979/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:838565/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:822905/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:815185/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:556961/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:776197/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:765484/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:7571/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:736866/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:727150/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:703941/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:636215/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:602370/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1785778/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:572703/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1165801/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1342713/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1336749/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1333190/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1319966/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1303434/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1280340/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1257744/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1237990/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:122605/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:120041/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1170812/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1344622/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1141228/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1134761/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1074612/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1072241/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1071515/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1070909/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1044781/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1033778/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1020336/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1007079/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1005239/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1616728/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:181080/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:100125/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1735246/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1720868/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1690622/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1667548/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1666771/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1645582/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:164543/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:164168/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1634103/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1899040/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1601345/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1590048/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1562864/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1533822/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1529062/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1527085/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1471822/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1452385/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1447867/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTa  >  1:1414898/1‑62 (MQ=255)
cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGAGGCAGGTGGACTTCTa  >  1:389400/1‑62 (MQ=255)
                                   |                          
CAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTA  >  minE/2694223‑2694284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A