Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,032,071 |
+CG |
coding (1896/2199 nt) |
zntA ← |
zinc, cobalt and lead efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,032,066 | 1 | . | G | 100.0%
| 64.2
/ NA
| 19 | P634? (CCA→CNA) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (19/0); total (19/0) |
| * | W3110S.gb | 4,032,066 | 2 | . | C | 100.0%
| 65.5
/ NA
| 19 | P634R (CCA→CGA) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (19/0); total (19/0) |
TCCCGATGGCGGCAGCTTTCATCGCTG‑‑GCGCGTCGTTAATACCGTCACCGACCATCGCCAGCG > W3110S.gb/4032040‑4032102
||
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATAcc > 1:1356275/1‑45 (MQ=25)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:996695/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:718483/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:672026/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:648203/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:578737/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:507991/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:305862/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:288577/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:2513513/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:2338783/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:2148151/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:2040431/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:2007508/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:1393842/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgcg > 1:1367992/1‑65 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgc > 1:2326216/1‑64 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgc > 1:645296/1‑64 (MQ=38)
tCCCGATGGCGGCAGCTTTCATCGCTGGCGCGCGTCGTTAATACCGTCACCGACCATCGCCAgc > 1:1346848/1‑64 (MQ=38)
||
TCCCGATGGCGGCAGCTTTCATCGCTG‑‑GCGCGTCGTTAATACCGTCACCGACCATCGCCAGCG > W3110S.gb/4032040‑4032102
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
