Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R1 178 99.0 1887089 95.3% 1798395 63.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 761,994 +AT coding (51/1218 nt) sucB → dihydrolipoyltranssuccinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb761,9921.A100.0% 396.8 / NA 106D17N (GAT→AAT) sucBdihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (106/0);  total (106/0)
*W3110S.gb761,9922.T100.0% 404.8 / NA 106D17Y (GAT→TAT) sucBdihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (106/0);  total (106/0)

Only 100 of 106 total aligned reads displayed.

ATGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCG‑‑ATGCCACCGTCGCA  >  W3110S.gb/761944‑762006
                                                 ||              
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCCCCGTCGCa  >  1:1573126/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGc   >  1:1587804/1‑64 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:341065/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:544741/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:530448/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:515945/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:508917/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:486092/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:467460/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:462774/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:460910/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:448802/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:412001/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:406909/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:381204/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:544860/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:335075/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:249061/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:210174/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:201502/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:195058/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:191306/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:182893/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1812871/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1777476/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1776523/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:747012/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:950022/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:939511/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:93641/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:933226/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:926384/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:920783/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:895001/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:871759/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:81792/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:810936/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:776165/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:774436/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1733545/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:712403/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:711697/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:695551/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:688341/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:658061/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:640129/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:624773/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:62474/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:617525/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:575646/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:574242/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1138055/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1351490/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1343681/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1298186/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1290980/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1273862/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1267537/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1260620/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1247264/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1228482/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1213517/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1171567/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1148369/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1416123/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1137578/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:113007/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1123169/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1118574/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1111482/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1102243/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1084473/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1061237/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1035850/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:103095/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1022885/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1568148/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1015901/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1725866/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1716191/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1701446/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1687028/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1680003/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1640619/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1631467/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1628797/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1599647/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1569620/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1747218/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1551532/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1551350/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1540998/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1513150/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1483680/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1478286/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1461112/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:143306/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1427059/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGTCGCa  >  1:1426314/1‑65 (MQ=255)
aTGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATATGCCACCGACGCa  >  1:1430540/1‑65 (MQ=255)
                                                 ||              
ATGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCG‑‑ATGCCACCGTCGCA  >  W3110S.gb/761944‑762006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A