Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R1 178 99.0 1887089 95.3% 1798395 63.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,274,151 Δ1 bp coding (322/417 nt) yjbQ → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,274,1510C.100.0% 715.2 / NA 159coding (322/417 nt)yjbQconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (159/0);  total (159/0)

Only 100 of 159 total aligned reads displayed.

TCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCA  >  W3110S.gb/4274101‑4274166
                                                  |               
tCAAATCCTCAATGCTGGGACCCTCGCTTGTATTGACGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:290878/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGCCCGGCa  >  1:1530078/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGc   >  1:1831044/1‑64 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGc   >  1:1789056/1‑64 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGc   >  1:649619/1‑64 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGc   >  1:645980/1‑64 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:415434/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:490259/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:406832/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:399101/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:389464/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:389000/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:373831/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:365019/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:330917/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:322347/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:320750/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:305446/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:292265/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:283927/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:230302/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:21332/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:198522/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:195101/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1873423/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1844889/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1841068/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:681149/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1029548/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:977037/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:955327/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:943640/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:909361/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:896706/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:886722/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:843878/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:798504/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:786862/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:758217/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:474791/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:670060/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:61454/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:608765/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:603352/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:571175/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:563306/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:534817/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:516442/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:497558/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:494298/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:98472/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1186099/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1419151/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1379084/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1333526/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1332184/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1301444/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1293435/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1274841/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1268087/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1250580/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1222405/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1205981/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:142166/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1182374/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1161151/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1155755/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1150502/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1135344/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1134965/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1127059/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1099483/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1085400/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1074469/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:104518/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1615961/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1826803/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1800107/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1777356/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1751747/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1751605/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1728793/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1704977/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1660574/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1647904/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1637298/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1839257/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1606715/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1597558/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1594344/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1579090/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1501711/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1492611/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1482923/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:145667/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1446610/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1434040/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCAGCa  >  1:1817896/1‑65 (MQ=255)
tCAAATCCTCAATGCTGGGAACATAGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:47297/1‑65 (MQ=255)
tCAAATCCTCAAAGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGG‑GTATTCAGACCGGCa  >  1:1113427/1‑65 (MQ=255)
                                                  |               
TCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCA  >  W3110S.gb/4274101‑4274166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A