Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,042,971 |
G→A |
A39T (GCC→ACC) |
livK → |
leucine transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,042,971 | 0 | G | A | 100.0%
| 128.6
/ NA
| 38 | A39T (GCC→ACC) | livK | leucine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (4/34); total (4/34) |
ACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGA > W3110S.gb/4042912‑4043020
|
acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg > 1:3015034/1‑65 (MQ=255)
acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg > 1:110378/1‑65 (MQ=255)
acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg > 1:1954878/1‑65 (MQ=255)
acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg > 1:817839/1‑65 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:2965/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:2764609/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:2606892/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:2097809/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:1986165/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:1757786/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:1665496/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:1637808/65‑1 (MQ=255)
ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:1311859/65‑1 (MQ=255)
ggCGCGATGTCCGGCACGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt < 1:230858/65‑1 (MQ=255)
gATGTCCGGCCCGATTACCCAGT‑GGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:2410377/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:817521/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:55494/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:501743/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:85244/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:448865/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:875654/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:423001/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:365903/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:1030722/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:924682/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:2886306/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:2784543/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:2774537/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:2621040/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:2221898/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:1998669/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:1899692/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:1822034/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:1519894/65‑1 (MQ=255)
aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGGCAGGCAATTAAAGa < 1:1585869/65‑1 (MQ=255)
tGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGTCAATTAAAGa < 1:107217/64‑1 (MQ=255)
tGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:514298/64‑1 (MQ=255)
tGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa < 1:1701947/64‑1 (MQ=255)
|
ACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGA > W3110S.gb/4042912‑4043020
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
