Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,431,950 |
A→G |
V52A (GTT→GCT) |
yjfZ ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,431,950 | 0 | A | G | 100.0%
| 72.9
/ NA
| 21 | V52A (GTT→GCT) | yjfZ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/4); total (17/4) |
CTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGT > W3110S.gb/4431893‑4432002
|
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:2554662/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:40016/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:338458/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:2899123/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:285137/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:2838130/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:276554/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:261714/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:1026093/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:2327313/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:2120327/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:1441568/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:1304518/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:1132682/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg > 1:1048204/1‑65 (MQ=255)
cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTACTTTGTGGATCTCAGCAGTAc > 1:1598532/1‑64 (MQ=255)
cTCGTCGCTAACTTATT‑AATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACGt > 1:2640778/1‑65 (MQ=255)
tgtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt < 1:1852889/64‑1 (MQ=255)
tgtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt < 1:177783/64‑1 (MQ=255)
tgtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt < 1:356979/64‑1 (MQ=255)
gtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt < 1:2628542/63‑1 (MQ=255)
|
CTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGT > W3110S.gb/4431893‑4432002
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
