Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,763,764 Δ1 bp noncoding (80/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,763,7640G.100.0% 16.1 / NA 39noncoding (80/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/39);  total (0/39)

CATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATC  >  minE/1763722‑1763817
                                          |                                                     
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:663113/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:177187/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:190928/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:230091/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:318786/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:342943/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:350950/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:413511/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:422890/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:483907/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:503469/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:613234/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:613558/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:307597/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:694970/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:738556/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:743007/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:774262/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:800131/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:812711/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:816414/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:879245/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:884642/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:994722/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:9997/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1361268/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1218024/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1208455/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1198768/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1281600/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1283422/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1091134/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1415950/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1445006/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1035923/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1047380/58‑1 (MQ=2)
taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1492900/58‑1 (MQ=2)
     cTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1498990/54‑1 (MQ=2)
         cccGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg                                 <  1:1082571/50‑1 (MQ=2)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAAt   >  1:1232390/1‑54 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:931527/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1010567/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:668178/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1018204/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:706736/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:992780/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1140863/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:923589/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:589687/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1081173/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1062548/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:644289/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1276479/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:561341/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:522908/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:47642/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:452666/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1343788/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:358740/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:142291/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1005789/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:265246/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1449302/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:227485/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1504531/1‑55 (MQ=33)
                                         tgctTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc  >  1:1499520/1‑55 (MQ=33)
                                         tgctTCCTGCTACCGTTCGACTTGCATGTGTTAGGCCTgccgc              >  1:149874/1‑43 (MQ=255)
                                          |                                                     
CATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATC  >  minE/1763722‑1763817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A