Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
770,702 |
G→A |
100% |
I180I (ATC→ATT) |
purB ← |
adenylosuccinate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 770,702 | 0 | G | A | 100.0%
| 58.4
/ NA
| 18 | I180I (ATC→ATT) | purB | adenylosuccinate lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/18); total (0/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACC > minE/770678‑770728
|
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGTCTGGCTGAcc < 1:770930/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:222765/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:887593/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:804467/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:625089/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:465355/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:33321/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:2267843/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:2255078/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:1114850/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:216984/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:2046518/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:196998/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:1883593/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:1791110/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:1708766/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:1691795/51‑1 (MQ=255)
ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc < 1:1439549/51‑1 (MQ=255)
|
GGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACC > minE/770678‑770728
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
