Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,753,336 |
T→C |
T35A (ACG→GCG) |
ydhW ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,753,336 | 0 | T | C | 100.0%
| 116.6
/ NA
| 33 | T35A (ACG→GCG) | ydhW | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/19); total (14/19) |
TGCCCAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGTATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTC > W3110S.gb/1753269‑1753390
|
tGCCCAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCAt > 1:4073613/1‑70 (MQ=255)
tGCCCAGACGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCAt > 1:1926564/1‑70 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:131648/71‑1 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:1330994/71‑1 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:3825556/71‑1 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:3805940/71‑1 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:3099410/71‑1 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:2131825/71‑1 (MQ=255)
ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc < 1:1856051/71‑1 (MQ=255)
cGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGt > 1:1591982/1‑71 (MQ=255)
aTGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCa < 1:760000/71‑1 (MQ=255)
aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa < 1:754761/71‑1 (MQ=255)
aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa < 1:2015760/71‑1 (MQ=255)
aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa < 1:1496148/71‑1 (MQ=255)
aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa < 1:3542458/71‑1 (MQ=255)
aTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa < 1:1370353/70‑1 (MQ=255)
aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc < 1:1078064/57‑1 (MQ=255)
aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc < 1:677836/57‑1 (MQ=255)
aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc < 1:226754/57‑1 (MQ=255)
aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc < 1:3428298/57‑1 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:1876011/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:425746/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:1531278/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:3589532/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:3096324/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:1790943/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:2123341/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:1869716/1‑71 (MQ=255)
tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg > 1:1874550/1‑71 (MQ=255)
ttCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAAc < 1:2544435/55‑1 (MQ=255)
gTTCTGGTTCGGTTTCCGCATCAACGGGGTGACGCa < 1:1734504/36‑1 (MQ=37)
tCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTc > 1:3395894/1‑71 (MQ=255)
tCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTc > 1:2085092/1‑71 (MQ=255)
|
TGCCCAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGTATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTC > W3110S.gb/1753269‑1753390
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
