Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,103,301 |
A→G |
R102R (CGT→CGC) |
greB ← |
transcription elongation factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,103,301 | 0 | A | G | 100.0%
| 222.4
/ NA
| 62 | R102R (CGT→CGC) | greB | transcription elongation factor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/26); total (36/26) |
GGGGAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAACGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTG > W3110S.gb/4103239‑4103366
|
ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt < 1:3680535/71‑1 (MQ=255)
ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt > 1:1091496/1‑71 (MQ=255)
ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt > 1:3844804/1‑71 (MQ=255)
ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt < 1:4064224/71‑1 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCAc > 1:921828/1‑68 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:4137715/1‑70 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:3040676/1‑70 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:2814685/1‑70 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:2705810/1‑70 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:1084124/1‑70 (MQ=255)
tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:1210916/1‑70 (MQ=255)
gaTGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATcgtcgt < 1:2523430/71‑1 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCa > 1:3011013/1‑53 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:12472/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:3947188/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:3114584/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:3996126/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:2871795/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:1624322/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:4001097/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:2604922/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:2445962/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:2184004/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg > 1:1884332/1‑64 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGtttt > 1:3962504/1‑68 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGtttt > 1:784949/1‑68 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGttt > 1:1408219/1‑67 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGttt > 1:2879180/1‑67 (MQ=255)
aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCa < 1:385478/70‑1 (MQ=255)
tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtct > 1:2253968/1‑71 (MQ=255)
tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtct > 1:1195992/1‑71 (MQ=255)
tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc > 1:1544829/1‑70 (MQ=255)
tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc > 1:2859744/1‑70 (MQ=255)
tttACGGCCAAAAATTTCATCGAAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGtt > 1:1556580/1‑63 (MQ=255)
tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc < 1:2176211/68‑1 (MQ=255)
tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc < 1:2945981/68‑1 (MQ=255)
tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc < 1:3336798/68‑1 (MQ=255)
tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc < 1:2186045/68‑1 (MQ=255)
tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc < 1:2247806/68‑1 (MQ=255)
tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAta < 1:3895117/68‑2 (MQ=255)
tACGGCCAAAAATTTCATCGAAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc < 1:3757556/68‑1 (MQ=255)
gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:445299/1‑47 (MQ=255)
gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:931851/1‑47 (MQ=255)
gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:3657689/1‑47 (MQ=255)
gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:353967/1‑47 (MQ=255)
gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:288194/1‑47 (MQ=255)
gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc > 1:1898014/1‑47 (MQ=255)
aTCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCaaaa < 1:3900971/70‑1 (MQ=255)
cGACAATTCGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCTAAAAAGACTTTg < 1:509876/71‑1 (MQ=255)
cGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTg < 1:3877798/71‑1 (MQ=255)
cGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTg < 1:3468438/71‑1 (MQ=255)
cGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTg < 1:2717723/71‑1 (MQ=255)
aaTACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt < 1:2945753/71‑1 (MQ=255)
aaTACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt < 1:2573427/71‑1 (MQ=255)
aaTACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt < 1:2570000/71‑1 (MQ=255)
tACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt < 1:1849428/69‑1 (MQ=255)
cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg < 1:1075376/71‑1 (MQ=255)
cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg < 1:914985/71‑1 (MQ=255)
cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg < 1:1905205/71‑1 (MQ=255)
cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg < 1:3155374/71‑1 (MQ=255)
cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg < 1:3564438/71‑1 (MQ=255)
cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg < 1:3612716/71‑1 (MQ=255)
|
GGGGAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAACGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTG > W3110S.gb/4103239‑4103366
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
