Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,428,265 |
+G |
coding (738/855 nt) |
ulaE → |
L‑xylulose 5‑phosphate 3‑epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,428,262 | 1 | . | G | 100.0%
| 175.1
/ NA
| 48 | C245W (TGC→TGG) | ulaE | L‑xylulose 5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (8/40); total (8/40) |
CCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGA > W3110S.gb/4428197‑4428303
|
ccGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑ggggc > 1:3053074/1‑69 (MQ=255)
gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga < 1:2550784/71‑1 (MQ=255)
gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga < 1:2097794/71‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:970822/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:2931226/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:3184775/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:3527896/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:3604063/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:3632749/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:376339/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:3990818/70‑1 (MQ=255)
tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:807164/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAGACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:3640687/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:352664/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:3415512/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:664739/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:1292267/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:2920433/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:2739016/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:1374653/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:1587520/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:152183/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:2341999/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:23825/70‑1 (MQ=255)
gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:2670250/70‑1 (MQ=255)
gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa < 1:213597/71‑1 (MQ=255)
gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa < 1:222044/71‑1 (MQ=255)
gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa < 1:3051710/71‑1 (MQ=255)
gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa < 1:2234664/71‑1 (MQ=255)
tttCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGc > 1:3846693/1‑71 (MQ=255)
aaCGTTGTTTCGAATCGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga < 1:93637/56‑1 (MQ=255)
tgttTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg < 1:2520543/55‑1 (MQ=255)
tttCGAAACGCTGAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:2849487/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:3908627/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:3929452/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:4088060/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:474511/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:3471366/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:3374734/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:56013/52‑1 (MQ=255)
tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt < 1:3059805/52‑1 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:750507/1‑52 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:1028045/1‑52 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:328097/1‑52 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:2725008/1‑52 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:3132254/1‑52 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:3075606/1‑52 (MQ=255)
tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:2898369/1‑52 (MQ=255)
cGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGa < 1:2909021/70‑1 (MQ=255)
|
CCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGA > W3110S.gb/4428197‑4428303
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
