Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,308,687 |
G→A |
34.3% |
I464I (ATC→ATT) |
yjcS ← |
predicted alkyl sulfatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,308,687 | 0 | G | A | 34.3%
| 37.6
/ 30.8
| 35 | I464I (ATC→ATT) | yjcS | predicted alkyl sulfatase |
| Reads supporting (aligned to +/- strand): ref base G (21/2); new base A (2/10); total (23/12) |
| Fisher's exact test for biased strand distribution p-value = 2.03e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 |
ACCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAT > W3110S.gb/4308622‑4308754
|
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtgatga < 1:693/70‑1 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1802853/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1677081/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:955820/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1072977/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:908937/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1139564/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1899474/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:695927/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:508714/1‑70 (MQ=255)
aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:377466/1‑70 (MQ=255)
cTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGcc > 1:403140/1‑70 (MQ=255)
gTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGt < 1:433610/71‑1 (MQ=255)
cACCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGACGAGCGCCTGCACGTAACGt > 1:1101062/1‑71 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt > 1:102523/1‑71 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt > 1:1165509/1‑71 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt > 1:100918/1‑71 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:390320/71‑1 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1951422/71‑1 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:972826/71‑1 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1744583/71‑1 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1681212/71‑1 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1488266/71‑1 (MQ=255)
aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1549258/71‑1 (MQ=255)
ccTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1349535/70‑1 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGACCCGCCCAGCGCCTGCACGTAACGTTTa > 1:1652230/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGttt > 1:233069/1‑70 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1066352/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:477044/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1313336/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1461002/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:249628/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1572794/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1297295/1‑71 (MQ=255)
cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1785980/1‑71 (MQ=255)
cGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTcc < 1:307419/70‑1 (MQ=255)
cGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTcc < 1:526482/70‑1 (MQ=255)
cgcCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCa < 1:1755373/70‑1 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCCTCTCCACCTGACCATACGGAt > 1:1390311/1‑71 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:607115/1‑71 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:1850528/1‑71 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:759781/1‑71 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:10800/1‑71 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:1686939/1‑71 (MQ=255)
gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:1038937/1‑71 (MQ=255)
gTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:541293/1‑71 (MQ=255)
|
ACCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAT > W3110S.gb/4308622‑4308754
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
