Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
408,996 |
G→A |
A83V (GCG→GTG) |
rdgC ← |
DNA‑binding protein, non‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 408,996 | 0 | G | A | 100.0%
| 107.2
/ NA
| 36 | A83V (GCG→GTG) | rdgC | DNA‑binding protein, non‑specific |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/24); total (12/24) |
AATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCA > W3110S.gb/408928‑409061
|
aaTCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCAcc < 1:3222431/71‑1 (MQ=255)
cTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCAcc > 1:135596/1‑68 (MQ=255)
tttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt < 1:3112743/70‑1 (MQ=255)
tttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt < 1:3107413/70‑1 (MQ=255)
tttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt < 1:3067980/70‑1 (MQ=255)
ttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa < 1:1091455/70‑1 (MQ=255)
ttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa < 1:1210372/70‑1 (MQ=255)
ttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa < 1:282686/70‑1 (MQ=255)
cGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAAt < 1:943411/70‑1 (MQ=255)
cttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGga < 1:4391305/70‑1 (MQ=255)
cttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGga < 1:2044786/70‑1 (MQ=255)
cttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGga < 1:2306210/70‑1 (MQ=255)
tACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGggag > 1:2987962/1‑70 (MQ=255)
ccTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGAttttt < 1:20047/71‑1 (MQ=255)
cTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt < 1:3319135/45‑1 (MQ=255)
ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAAt < 1:1443109/45‑1 (MQ=255)
ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAAt < 1:1526217/45‑1 (MQ=255)
ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATc < 1:2526994/46‑1 (MQ=255)
ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATc < 1:2779431/46‑1 (MQ=255)
tCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTttcttc > 1:4073900/1‑71 (MQ=255)
ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTaa > 1:2134815/1‑42 (MQ=255)
ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTtcttct > 1:102235/1‑71 (MQ=255)
ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTtcttct > 1:1548515/1‑71 (MQ=255)
gTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg < 1:2417395/71‑1 (MQ=255)
ttGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGgaga > 1:3328629/1‑41 (MQ=255)
ttGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg > 1:915602/1‑69 (MQ=255)
tGGCGATTTTCGCTTCCAGCACCTGTTTATTCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg > 1:408101/1‑68 (MQ=255)
tGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg > 1:2108089/1‑68 (MQ=255)
tCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc > 1:1746563/1‑70 (MQ=255)
ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc < 1:1337746/69‑1 (MQ=255)
ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc < 1:1239983/69‑1 (MQ=255)
ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc < 1:2252709/69‑1 (MQ=255)
ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc < 1:1750618/69‑1 (MQ=255)
ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCa > 1:3141611/1‑71 (MQ=255)
ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGAGCAGATAACAATTTGAc < 1:4515093/69‑1 (MQ=255)
cAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCa < 1:2324133/70‑1 (MQ=255)
|
AATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCA > W3110S.gb/408928‑409061
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
