Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,111,845 |
A→C |
L702R (CTG→CGG) |
yrfF ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,111,845 | 0 | A | C | 100.0%
| 222.2
/ NA
| 65 | L702R (CTG→CGG) | yrfF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (26/39); total (26/39) |
TGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCAGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGT > W3110S.gb/4111777‑4111911
|
tGCGCGACAGGGTAGGATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt > 1:3881066/1‑71 (MQ=255)
tGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt > 1:3585897/1‑71 (MQ=255)
tGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt > 1:262923/1‑71 (MQ=255)
tGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt > 1:2324106/1‑71 (MQ=255)
aGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGtt < 1:2737234/71‑1 (MQ=255)
aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc > 1:4627798/1‑71 (MQ=255)
aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc > 1:3988841/1‑71 (MQ=255)
aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc > 1:3239806/1‑71 (MQ=255)
aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc > 1:29272/1‑71 (MQ=255)
tAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCt > 1:3166709/1‑71 (MQ=255)
acgtGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCATTGGGGGTTCAGGCAGCttt > 1:1214679/4‑71 (MQ=255)
aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt > 1:994867/1‑71 (MQ=255)
aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt > 1:566088/1‑71 (MQ=255)
aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt > 1:4268952/1‑71 (MQ=255)
aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt > 1:3048479/1‑71 (MQ=255)
aCCTGCCCCGCAAACGTGTTATTCGATAAGTCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGTAACttt > 1:1950332/1‑71 (MQ=38)
cTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCa < 1:4216643/71‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:738620/71‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:4527722/71‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:426187/71‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:1575054/71‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCata < 1:3579963/70‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat < 1:1720814/69‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat < 1:3891741/69‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat < 1:3764579/69‑1 (MQ=255)
ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat < 1:4418976/69‑1 (MQ=255)
aCGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat < 1:4135098/61‑1 (MQ=255)
cGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTAATAATAGGCCTg > 1:3044570/1‑70 (MQ=255)
cGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGGTCAGGCAGCTTTCATAATAGGCCTGa < 1:86691/71‑1 (MQ=255)
ttATTCGATAAGGCTTTCTGAAGTGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:1528187/58‑1 (MQ=255)
aTTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAAtctc > 1:3558004/1‑70 (MQ=255)
aTTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAAtctc > 1:3690239/1‑70 (MQ=255)
cGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCcat < 1:1988353/70‑1 (MQ=255)
cGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCcat < 1:1350919/70‑1 (MQ=255)
aTAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat < 1:2394995/49‑1 (MQ=255)
aTAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCcatcat < 1:3258559/71‑1 (MQ=255)
tAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:374184/50‑1 (MQ=255)
tAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa < 1:108928/50‑1 (MQ=255)
aGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataata > 1:3404152/1‑50 (MQ=255)
aGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGc < 1:3340571/70‑1 (MQ=255)
aGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCg < 1:398244/71‑1 (MQ=255)
cTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt < 1:904352/40‑1 (MQ=255)
ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGaa > 1:498615/1‑53 (MQ=255)
ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGaa > 1:2880214/1‑53 (MQ=255)
ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGaa > 1:4621112/1‑53 (MQ=255)
ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc > 1:2248119/1‑71 (MQ=255)
tCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc < 1:1716854/70‑1 (MQ=255)
tCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc < 1:1604826/70‑1 (MQ=255)
aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCTTAATAGGCCTGAATCTCCATCATGCGAGTGCGAt < 1:1847271/69‑1 (MQ=255)
aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt < 1:3739911/69‑1 (MQ=255)
aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt < 1:2659309/69‑1 (MQ=255)
aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt < 1:615844/69‑1 (MQ=255)
atgggTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc < 1:44188/63‑1 (MQ=255)
aGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCg < 1:2105223/60‑1 (MQ=255)
aGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt < 1:3612997/68‑1 (MQ=255)
aGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGAc < 1:4369091/71‑1 (MQ=255)
gggTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc < 1:208971/63‑1 (MQ=255)
gggTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTACATCATGCGAGTGc < 1:3994929/63‑1 (MQ=255)
gTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTg > 1:3184349/1‑71 (MQ=255)
gTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTg > 1:3384335/1‑71 (MQ=255)
gATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTgg > 1:965819/1‑70 (MQ=255)
aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGc < 1:2220695/52‑1 (MQ=255)
aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGt < 1:3192470/70‑1 (MQ=255)
aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGt < 1:3532730/70‑1 (MQ=255)
aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGt < 1:3611191/70‑1 (MQ=255)
|
TGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCAGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGT > W3110S.gb/4111777‑4111911
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
