Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,174,189 |
C→T |
intergenic (+20/+9) |
bfr → / ← gspO |
bacterioferritin, iron storage and detoxification protein/bifunctional prepilin leader peptidase and methylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,174,189 | 0 | C | T | 100.0%
| 113.0
/ NA
| 40 | intergenic (+20/+9) | bfr/gspO | bacterioferritin, iron storage and detoxification protein/bifunctional prepilin leader peptidase and methylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/27); total (13/27) |
GATGGGCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAACCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGCCAC > W3110S.gb/4174121‑4174255
|
gaTGGGCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAAtcc < 1:271734/71‑1 (MQ=255)
gggCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAAtcctcc > 1:771158/1‑71 (MQ=255)
gggCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAAtcctcc > 1:3969521/1‑71 (MQ=255)
gggCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAAtcctcc > 1:4502009/1‑71 (MQ=255)
gCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCg > 1:498211/1‑71 (MQ=255)
cgtGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCgg < 1:2450428/69‑1 (MQ=255)
cAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTtaa > 1:3049099/1‑71 (MQ=255)
aaaaTTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTtaat < 1:642428/71‑1 (MQ=255)
aaTTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTtaataa < 1:3787822/71‑1 (MQ=255)
tATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTtaataa > 1:3584795/1‑68 (MQ=255)
aTCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTtaataa > 1:2518628/1‑67 (MQ=255)
aTCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAagag < 1:2260040/70‑1 (MQ=255)
aTCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAagag < 1:3534188/70‑1 (MQ=255)
tCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAgaga < 1:153478/70‑1 (MQ=255)
cAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATc < 1:3113151/68‑1 (MQ=255)
aGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGc > 1:2842188/1‑71 (MQ=255)
gacaGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCa < 1:3202285/70‑1 (MQ=255)
cacaGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCa < 1:3084046/71‑1 (MQ=255)
acaGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAgaga < 1:4271256/61‑1 (MQ=255)
caGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGa < 1:789915/71‑1 (MQ=255)
caGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGa < 1:3048565/71‑1 (MQ=255)
caGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGa < 1:2785589/71‑1 (MQ=255)
caGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGa < 1:2790978/71‑1 (MQ=255)
aGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGa < 1:1059865/70‑1 (MQ=255)
gcgAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCa < 1:3048664/62‑1 (MQ=255)
gcgAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCa < 1:2616662/62‑1 (MQ=255)
gcgAAGAAGGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCa < 1:4155625/62‑1 (MQ=255)
gaagGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGtt > 1:1545493/1‑70 (MQ=255)
agGTTGAGCCGTGTCGGGATGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTAt < 1:3273112/71‑1 (MQ=255)
agGTTGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTAt < 1:585293/71‑1 (MQ=255)
tGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCAtg > 1:4611006/1‑71 (MQ=255)
tGAGCCGTGTCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCAtg > 1:4627477/1‑71 (MQ=255)
tgtCGGGAGGGAGAATCCTCCCGGTTAATAAGAGACCCAGGCCa < 1:4259644/44‑1 (MQ=255)
tCGGGAGGGAGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAgc > 1:2436626/1‑71 (MQ=255)
gggagggaGAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAgcg > 1:4160546/1‑70 (MQ=255)
gggagAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGc < 1:780428/71‑1 (MQ=255)
gggagAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGc < 1:1066908/71‑1 (MQ=255)
gggagAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGc < 1:4047865/71‑1 (MQ=255)
gggagAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGc < 1:1844946/71‑1 (MQ=255)
ggagAATCCTCCCGGTTAATAAGAGATCCAGGCCAGa < 1:4313524/37‑1 (MQ=255)
ggagAATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCa < 1:2529532/67‑1 (MQ=255)
agaATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGCCa > 1:2583608/1‑70 (MQ=255)
agaATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGCCa > 1:2950390/1‑70 (MQ=255)
agaATCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGCCAc > 1:1089451/1‑71 (MQ=255)
|
GATGGGCCTGCAAAATTATCTGCAAGCACAGATCCGCGAAGAAGGTTGAGCCGTGTCGGGAGGGAGAACCCTCCCGGTTAATAAGAGATCCAGGCCAGATACCCCAGGTTTATCATGCTGCCGAGCGCAAGCCAC > W3110S.gb/4174121‑4174255
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
