Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,313,148 |
(G)5→6 |
coding (11/696 nt) |
alsE ← |
allulose‑6‑phosphate 3‑epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,313,143 | 1 | . | G | 96.3%
| 93.7
/ ‑2.8
| 27 | coding (16/696 nt) | alsE | allulose‑6‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (26/0); total (27/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑GGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGTCAAGGGCGACAGCCGCGAT > W3110S.gb/4313087‑4313207
|
gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:1136845/60‑1 (MQ=255)
gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:313111/60‑1 (MQ=255)
gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:579750/60‑1 (MQ=255)
gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:703072/60‑1 (MQ=255)
gCTGTCGATAAATTCGATCTGTTCTTAAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:1772728/60‑1 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:752918/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:727832/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:770199/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:686355/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:793908/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:551663/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:537026/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:363265/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:337798/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:859279/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:247631/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:2363782/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1027948/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:2047848/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:2002759/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1798737/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1697290/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:166091/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1637637/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1530248/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:144582/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:130450/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1252973/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1245524/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1208078/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1178637/1‑64 (MQ=255)
tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑GGGGGAGAtttt > 1:964319/1‑65 (MQ=255)
a‑GGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGTCAAGGGCGACAGCCGCGAt > 1:2207125/1‑65 (MQ=255)
|
GCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑GGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGTCAAGGGCGACAGCCGCGAT > W3110S.gb/4313087‑4313207
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
