Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,395,175 |
(T)5→4 |
coding (1017/1053 nt) |
rsgA ← |
ribosome small subunit‑dependent GTPase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,395,171 | 0 | T | . | 100.0%
| 96.2
/ NA
| 23 | coding (1021/1053 nt) | rsgA | ribosome small subunit‑dependent GTPase A |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (23/0); total (23/0) |
TTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAA > W3110S.gb/4395132‑4395197
|
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTgc > 1:1483509/1‑49 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:2377934/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:994368/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:938239/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:779356/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:646255/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:645196/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:604604/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:553886/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:453746/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:435157/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:2525565/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:2463147/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1093192/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:2123610/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1776514/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1757393/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1699519/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1688332/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:161812/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1580624/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1547031/1‑65 (MQ=255)
ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa > 1:1242185/1‑65 (MQ=255)
|
TTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAA > W3110S.gb/4395132‑4395197
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
