Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,090,143 Δ2 bp coding (623‑624/702 nt) yhaK → predicted pirin‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,090,1420G.100.0% 457.4 / NA 95coding (622/702 nt)yhaKpredicted pirin‑related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (95/0);  total (95/0)
*minE2,090,1430C.100.0% 457.4 / NA 95coding (623/702 nt)yhaKpredicted pirin‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (95/0);  total (95/0)

TTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGC  >  minE/2090089‑2090161
                                                     ||                  
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACgggg   >  1:18453/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAgg   >  1:773194/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAgg   >  1:136167/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAgg   >  1:1299796/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAgg   >  1:1231678/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAgg   >  1:722934/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAgg   >  1:1083581/1‑70 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAg    >  1:1155771/1‑69 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:518207/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:29642/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:57458/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:555804/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:551954/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:330391/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:53286/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:526242/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:522903/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:331627/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:345037/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:404334/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:490307/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:672491/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:481495/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:46396/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:449256/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:431778/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:407415/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:397678/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:775790/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:974015/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:965870/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:963431/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:94442/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:916651/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:889171/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:869132/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:862611/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:855416/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:825317/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:576089/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:773646/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:761855/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:760096/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:700386/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:682044/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:677982/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:67253/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:230384/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:605942/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:599121/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1118691/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1342411/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1326286/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1317107/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1294946/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1273046/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1252355/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1219792/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1190918/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:11754/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1137102/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:11354/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1355218/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:111366/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1086659/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1085278/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1083973/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1070919/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1046707/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1027823/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1027205/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1017708/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1015997/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1361561/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:256746/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:247695/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1015490/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:217129/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:211097/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:191756/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:17284/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:172610/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:168516/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:16571/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1557163/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1556657/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1542278/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1532172/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1524502/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1482833/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1467647/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1443077/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:1394302/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:293311/1‑71 (MQ=255)
ttCATGCGCTTACGCATCATGAAGAGAAAGAGGCGCTGACCTGCGGTGATGGG‑‑GTTTATTCGTGACGAGGc  >  1:48131/1‑71 (MQ=255)
                                                     ||                  
TTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGC  >  minE/2090089‑2090161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A