Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F8 I0 R2 422 46.8 3726540 94.7% 3529033 61.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,177,469 G→A 100% A9V (GCG→GTG)  gspK ← general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,177,4690GA85.0% 95.3 / 10.9 40A9V (GCG→GTG) gspKgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base A (22/12);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 6.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGTCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGACTCT  >  W3110S.gb/4177415‑4177532
                                                      |                                                               
aTGTCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCgttgtt                                                  <  1:2644954/70‑1 (MQ=255)
   tCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCat                                               <  1:2697740/70‑1 (MQ=255)
   tCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCat                                               <  1:1825800/70‑1 (MQ=255)
       ctgcCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt                                           <  1:1171641/70‑1 (MQ=255)
       ctgcCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt                                           <  1:3298341/70‑1 (MQ=255)
                  gcCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTtattat                               <  1:845982/71‑1 (MQ=255)
                   ccATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt                                           <  1:1001653/58‑1 (MQ=255)
                   ccATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt                                           <  1:262659/58‑1 (MQ=255)
                   ccATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt                                           <  1:1329343/58‑1 (MQ=255)
                        aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat                                  >  1:2780493/1‑62 (MQ=255)
                        aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat                                  >  1:2708545/1‑62 (MQ=255)
                        aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat                                  >  1:3647239/1‑62 (MQ=255)
                        aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat                                  >  1:1249790/1‑62 (MQ=255)
                        aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat                                  >  1:2364125/1‑62 (MQ=255)
                               gTAACATGAGTACAATGAGCAGCACGACGCCGCgttgtt                                                  <  1:2272637/39‑1 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt                                                  >  1:3599384/1‑37 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt                                                  >  1:879277/1‑37 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt                                                  >  1:1068183/1‑37 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt                                                  >  1:1743801/1‑37 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt                >  1:2480123/1‑71 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt                >  1:214628/1‑71 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt                >  1:1965189/1‑71 (MQ=255)
                                 aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt                >  1:1162461/1‑71 (MQ=255)
                                  ctgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg               <  1:2315897/68‑1 (MQ=255)
                                  ctgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg               <  1:1298804/68‑1 (MQ=255)
                                  ctgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg               <  1:2974220/68‑1 (MQ=255)
                                  aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa                                                >  1:1357602/1‑38 (MQ=255)
                                  aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa                                                >  1:1270198/1‑38 (MQ=255)
                                  aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa                                                >  1:3318116/1‑38 (MQ=255)
                                  aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa                                                >  1:3413629/1‑38 (MQ=255)
                                   tgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg               <  1:3090465/68‑1 (MQ=255)
                                      gAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCt           >  1:1349522/1‑71 (MQ=255)
                                       aGTACAATGAGCAGCACGACGCCGCGTTGTTCatta                                             >  1:1289343/1‑36 (MQ=255)
                                         tACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGc        <  1:2830/71‑1 (MQ=255)
                                         tACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGGCTGAGCTGGc        <  1:192607/71‑1 (MQ=255)
                                           cAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGa      <  1:327281/71‑1 (MQ=255)
                                           cAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGa      >  1:3625495/1‑71 (MQ=255)
                                              tGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGActc   >  1:2367404/1‑71 (MQ=255)
                                               gAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGActct  >  1:3022665/1‑71 (MQ=255)
                                               gAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGActct  >  1:2703765/1‑71 (MQ=255)
                                                      |                                                               
ATGTCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGACTCT  >  W3110S.gb/4177415‑4177532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A