Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
422,470 |
T→A |
100% |
S3S (TCA→TCT) |
asnB ← |
asparagine synthetase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 422,470 | 0 | T | A | 75.0%
| 191.5
/ 72.4
| 104 | S3S (TCA→TCT) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base T (0/26); new base A (0/78); total (0/104) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
Only 100 of 104 total aligned reads displayed.
CTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTG > minE/422410‑422520
|
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2220616/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:397280/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:363900/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:342725/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:337686/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:312750/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2296863/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2249785/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2244895/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2230789/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:400115/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2213162/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1051627/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2042628/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2040469/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1942379/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1926116/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1901912/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1875566/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1850879/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:645586/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:958662/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:917729/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:883983/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:879398/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:774176/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:731348/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:714680/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:678567/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:670681/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2155168/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:641512/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:591444/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:561130/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:525480/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:509194/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:469918/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:447949/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:410965/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1628858/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1502739/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1506794/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1522900/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1842768/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1498350/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1529565/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1539031/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1543385/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1460162/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1587738/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1458689/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1416698/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:168170/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1217517/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1069769/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1086014/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1811371/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:11200/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1800448/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1794663/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1171123/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1767247/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:130418/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1220191/67‑1 (MQ=255)
cTCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1242369/67‑1 (MQ=255)
tCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:27772/66‑1 (MQ=255)
tCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:643231/66‑1 (MQ=255)
tCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:505835/66‑1 (MQ=255)
tCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:351407/66‑1 (MQ=255)
tCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1621674/66‑1 (MQ=255)
tCGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:1636882/66‑1 (MQ=255)
cGAGTGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:2260166/65‑1 (MQ=255)
gCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATAGAacac < 1:359456/50‑1 (MQ=255)
cTCAACTGCGTCTGTTTTGATATGGAATACGCCAAAAATAGAacac < 1:1736359/46‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:665029/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1853663/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1867958/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:902108/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1091376/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1808744/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:804368/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1132008/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:760979/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1163265/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1983241/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:702899/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:2067711/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:344070/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1224522/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1691609/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:125194/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:2217343/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:55706/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1317587/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:2240633/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:2264675/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:2278487/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:285603/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:429127/66‑1 (MQ=255)
gAATACGCCAAAAATTGAACACATAACCTTCTACGTTAACCTGGTATTTGTTGCTTGTTGTGTTTg < 1:1740988/66‑1 (MQ=255)
|
CTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTG > minE/422410‑422520
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
