Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
810,589 |
T→G |
M275R (ATG→AGG) |
bioB → |
biotin synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 810,589 | 0 | T | G | 79.1%
| 120.8
/ 13.0
| 43 | M275R (ATG→AGG) | bioB | biotin synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base G (26/8); total (26/17) |
| Fisher's exact test for biased strand distribution p-value = 4.31e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 |
ATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGG > W3110S.gb/810525‑810654
|
atgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCTATGTGCtt < 1:1704722/71‑1 (MQ=255)
atgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCtt < 1:4017700/71‑1 (MQ=255)
atgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCtt < 1:384905/71‑1 (MQ=255)
atgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCtt < 1:2243889/71‑1 (MQ=255)
ggACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTc > 1:1431911/1‑66 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAggtg > 1:2039426/1‑37 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:293030/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:648828/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:563519/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:3366004/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:1077317/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:230322/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:2093817/1‑62 (MQ=255)
gACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtt > 1:204096/1‑62 (MQ=255)
cgAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:3512744/71‑1 (MQ=255)
cgAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:3913483/71‑1 (MQ=255)
cgAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:3209491/71‑1 (MQ=255)
cgAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:454175/71‑1 (MQ=255)
cgAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:2678802/71‑1 (MQ=255)
cgAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:281021/71‑1 (MQ=255)
gCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCaaa > 1:2607947/1‑70 (MQ=255)
gCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCaa > 1:3147513/1‑69 (MQ=255)
gCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTATACGGTTGCaaa > 1:3555484/1‑70 (MQ=255)
gaacgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGa > 1:3202159/1‑71 (MQ=255)
gaacgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGa > 1:3946608/1‑71 (MQ=255)
gaaagaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAActg > 1:2387007/1‑67 (MQ=255)
cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGcc > 1:1532011/1‑71 (MQ=255)
cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGcc > 1:1993294/1‑71 (MQ=255)
cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGcc > 1:181437/1‑71 (MQ=255)
cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGc > 1:397705/1‑70 (MQ=255)
cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGc > 1:1121430/1‑70 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:604154/71‑1 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:2260896/71‑1 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:3384466/71‑1 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:3284101/71‑1 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:2673578/71‑1 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:2615583/71‑1 (MQ=255)
ggCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg < 1:2525426/71‑1 (MQ=255)
gCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg > 1:3554053/1‑70 (MQ=255)
gCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg > 1:2160021/1‑70 (MQ=255)
gCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAAACCgg > 1:4030288/1‑70 (MQ=255)
cGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg > 1:3447105/1‑69 (MQ=255)
cGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg > 1:308406/1‑69 (MQ=255)
cGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg > 1:109200/1‑69 (MQ=255)
|
ATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGG > W3110S.gb/810525‑810654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
