Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F8 I0 R2	296	77.6	4260404	88.1%	3753415	61.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	447,412	G→A	100%	R102C (CGC→TGC)	kdpD ←	fused sensory histidine kinase in two‑component regulatory system with KdpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	447,412	0	G	A	81.0%	41.7 / 6.2	21	R102C (CGC→TGC)	kdpD	fused sensory histidine kinase in two‑component regulatory system with KdpE
Reads supporting (aligned to +/- strand): ref base G (1/3); new base A (5/12); total (6/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACGG  >  minE/447349‑447481

tGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCggcgggc                                                                 <  1:3773906/70‑1 (MQ=255)

     tGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGgcagcg                                                                    <  1:3209299/62‑1 (MQ=255)

     tGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGgcagcg                                                                    <  1:3010971/62‑1 (MQ=255)

     tGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGgcagcg                                                                    <  1:2455633/62‑1 (MQ=255)

            aaCCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATc                                                     >  1:1282074/1‑70 (MQ=255)

             aCCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAATATCAGCGCCGGGCGGCGGGCGAGggcggc                                                        <  1:2030142/66‑1 (MQ=255)

             aCCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATc                                                     <  1:2001751/69‑1 (MQ=255)

               cTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCga                                                   >  1:3545854/1‑69 (MQ=255)

                  gcgcATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCAGGGCGGCGGGCGAGGGCGGCATCGAGATCa                                              <  1:983885/71‑1 (MQ=255)

                       ttACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGggcagc                                                                     <  1:426626/43‑1 (MQ=255)

                         aCTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGc                                       >  1:1856608/1‑71 (MQ=255)

                             tgCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGa                                    <  1:3288517/70‑1 (MQ=255)

                                     ttCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATgccgcc                           >  1:364018/1‑71 (MQ=255)

                                        gTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCa                         <  1:3062706/70‑1 (MQ=255)

                                            aTTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCa                                              <  1:1808996/45‑1 (MQ=255)

                                            aTTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCa                                              <  1:1804088/45‑1 (MQ=255)

                                              tAAGATCAGCGCCGGGCAGCGGGCGAGGGCGTCATc                                                     <  1:709487/36‑1 (MQ=37)

                                              tAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCa                                              <  1:1723331/43‑1 (MQ=255)

                                               aaGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATc                                                     >  1:4258932/1‑35 (MQ=255)

                                               aaGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGc                 >  1:3468738/1‑71 (MQ=255)

                                                  aTCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTg              <  1:2657086/71‑1 (MQ=255)

                                                  aTCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTg              <  1:1870240/71‑1 (MQ=255)

                                                   gcAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTg              <  1:3066240/69‑1 (MQ=255)

                                                          cGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTa      <  1:1974320/71‑1 (MQ=255)

                                                          cGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTa      <  1:1751355/71‑1 (MQ=255)

                                                           gggCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTa      <  1:2469977/70‑1 (MQ=255)

                                                               ggcgggcgAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACgg  >  1:1527779/1‑70 (MQ=255)

TGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACGG  >  minE/447349‑447481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A