Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
470,819 |
A→G |
E320E (GAA→GAG) |
mdlB → |
fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 470,819 | 0 | A | G | 100.0%
| 329.4
/ NA
| 94 | E320E (GAA→GAG) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (40/54); total (40/54) |
TTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAG > W3110S.gb/470751‑470886
|
ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt < 1:4095127/71‑1 (MQ=255)
ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt < 1:2834416/71‑1 (MQ=255)
ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt < 1:3127115/71‑1 (MQ=255)
ttAATCGAACTGACCACGCAACACGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt < 1:3732400/71‑1 (MQ=255)
tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt > 1:3589089/1‑70 (MQ=255)
tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt > 1:3690433/1‑70 (MQ=255)
tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt > 1:181420/1‑70 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTg < 1:285535/70‑1 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:1156954/1‑71 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:758789/1‑71 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:2860707/1‑71 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:1457921/1‑71 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:617273/1‑71 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:4582389/1‑71 (MQ=255)
aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:1096771/1‑71 (MQ=255)
aTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGAt > 1:4613702/1‑71 (MQ=255)
tCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATg < 1:1095890/71‑1 (MQ=255)
gAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATgga > 1:1010922/1‑71 (MQ=255)
cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa > 1:4071046/1‑64 (MQ=255)
cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacgg < 1:1884713/71‑1 (MQ=255)
cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacg > 1:750901/1‑70 (MQ=255)
aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGATGGTGAGCGCGTGTTTGAGCTGATGGACGGAcc > 1:2324765/1‑71 (MQ=255)
aCCACGAAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGAcc > 1:600401/1‑71 (MQ=255)
cGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCgcgc > 1:1436701/1‑71 (MQ=255)
gCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGcc < 1:3977539/71‑1 (MQ=255)
cAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCgcgc > 1:3209318/1‑69 (MQ=255)
aCAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc < 1:1032232/71‑1 (MQ=255)
aCAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc < 1:3472695/71‑1 (MQ=255)
cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacgg < 1:770422/59‑1 (MQ=255)
cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc > 1:2490440/1‑70 (MQ=255)
cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc > 1:1336617/1‑70 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGGGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa > 1:350274/1‑71 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:689690/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:979996/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:363189/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:626449/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:3823550/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:439534/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:1282623/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:1581152/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:1466697/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:2097733/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:1875746/71‑1 (MQ=255)
aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:221625/71‑1 (MQ=255)
ggCGATGCTGCAACAGGCTGTTGTTGCTGGTGCGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa > 1:589499/1‑70 (MQ=255)
aTGCTGCAAAAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt < 1:3681926/44‑1 (MQ=38)
tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc < 1:1357336/71‑1 (MQ=255)
tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc < 1:1153065/71‑1 (MQ=255)
tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc < 1:4056170/71‑1 (MQ=255)
tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc < 1:1467650/71‑1 (MQ=255)
tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc < 1:4276549/71‑1 (MQ=255)
tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc < 1:3766995/71‑1 (MQ=255)
gctgcAACAGACTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc > 1:1811000/1‑70 (MQ=255)
cAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatg < 1:3332162/70‑1 (MQ=255)
cAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAat < 1:3534497/69‑1 (MQ=255)
aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:1488569/71‑1 (MQ=255)
aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:1467125/71‑1 (MQ=255)
aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgat > 1:673800/1‑70 (MQ=255)
aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgat > 1:3930806/1‑70 (MQ=255)
aCAGGCTGTTGCTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:4219831/71‑1 (MQ=255)
gCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCg > 1:606698/1‑71 (MQ=255)
tgttgtTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGcc > 1:2359134/1‑71 (MQ=255)
ttgttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:384684/63‑1 (MQ=255)
gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt < 1:3659337/70‑1 (MQ=255)
gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt > 1:1910874/1‑70 (MQ=255)
gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt > 1:3886615/1‑70 (MQ=255)
gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt < 1:737107/70‑1 (MQ=255)
gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt > 1:1856991/1‑70 (MQ=255)
gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt < 1:1600381/70‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:1995537/71‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:2172757/71‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:1343470/71‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:3388875/71‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:1309671/71‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:232526/71‑1 (MQ=255)
cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACa < 1:2780253/70‑1 (MQ=255)
gTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGt > 1:4288967/1‑71 (MQ=255)
gAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCATTACAGAGTgg > 1:2319872/1‑71 (MQ=255)
aGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATgatgat > 1:2846923/1‑53 (MQ=255)
aGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTgg > 1:3676376/1‑70 (MQ=255)
aGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTgg < 1:76212/70‑1 (MQ=255)
gcgcGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGACCGTTACag < 1:3955358/64‑1 (MQ=255)
cgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:2447175/61‑1 (MQ=255)
gtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAattatg < 1:3607387/44‑1 (MQ=38)
gtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:3308080/44‑1 (MQ=255)
tttGAGCTGATGGACTGACCGCGCCAGCAATATGGCAat < 1:56765/39‑1 (MQ=37)
tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAat < 1:4388207/39‑1 (MQ=255)
tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAat < 1:3156033/39‑1 (MQ=255)
tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATgatgat > 1:4485042/1‑45 (MQ=255)
tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCg > 1:2183352/1‑70 (MQ=255)
tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCg > 1:1134604/1‑70 (MQ=255)
tGAGCTGATGGACGGACCGCGCCAGCAATGTGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAg > 1:4208378/1‑71 (MQ=255)
tGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAg > 1:4543255/1‑71 (MQ=255)
tGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAg > 1:3249037/1‑71 (MQ=255)
|
TTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAG > W3110S.gb/470751‑470886
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
