Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
746,293 |
C→T |
A236V (GCC→GTC) |
ybgL → |
predicted lactam utilization protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 746,293 | 0 | C | T | 100.0%
| 124.7
/ NA
| 43 | A236V (GCC→GTC) | ybgL | predicted lactam utilization protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/27); total (16/27) |
CGGTCTGCCTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAT > W3110S.gb/746227‑746358
|
cGGTCTGCCTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGaa < 1:224883/71‑1 (MQ=255)
cctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGa > 1:806714/1‑71 (MQ=255)
aTGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGAttgtt < 1:4037975/71‑1 (MQ=255)
gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:3952794/1‑71 (MQ=255)
gCGACGGCCAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:4401427/1‑71 (MQ=255)
cTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGAtt < 1:3169078/71‑1 (MQ=255)
ggCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGaaaa > 1:1683117/1‑39 (MQ=255)
cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg > 1:4356421/1‑70 (MQ=255)
cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg > 1:3519595/1‑70 (MQ=255)
gACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGaaaaa < 1:2855813/71‑1 (MQ=255)
cTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg < 1:184930/71‑1 (MQ=255)
cTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg < 1:2064455/71‑1 (MQ=255)
tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAAccc > 1:3767295/1‑47 (MQ=255)
tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg > 1:980373/1‑70 (MQ=255)
tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg > 1:3550713/1‑70 (MQ=255)
tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg > 1:102121/1‑70 (MQ=255)
tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg > 1:4591794/1‑70 (MQ=255)
tACGCTCTGCATTT‑TCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGa > 1:3950697/1‑71 (MQ=255)
cGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAgg < 1:3874755/69‑1 (MQ=255)
cGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg < 1:2297908/68‑1 (MQ=255)
cGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGa > 1:2890958/1‑70 (MQ=255)
ctctGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGatat < 1:339924/71‑1 (MQ=255)
ctGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCa > 1:3548556/1‑71 (MQ=255)
tGCATTTGTCGAAAAGGGGATTGTTGTCTCAGCAt < 1:771683/35‑1 (MQ=37)
tGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCAt < 1:3739765/35‑1 (MQ=255)
tGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCAt < 1:136101/35‑1 (MQ=255)
tGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCAt < 1:666098/35‑1 (MQ=255)
gCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATa > 1:4400247/1‑35 (MQ=255)
gCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAAc > 1:1536708/1‑37 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:11079/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:3653255/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:54512/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:540203/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:4530905/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:1681550/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:2091310/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:2092420/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:2406056/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:241829/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:2813594/69‑1 (MQ=255)
cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:310886/69‑1 (MQ=255)
aTTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt < 1:4006185/71‑1 (MQ=255)
tttGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:640674/67‑1 (MQ=255)
|
CGGTCTGCCTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAT > W3110S.gb/746227‑746358
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
