Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F21 I0 R1	476	35.3	2812105	96.7%	2719305	61.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,918,430	G→A	8.0%	L264L (CTG→TTG)	insK ←	IS150 conserved protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,918,430	0	G	A	8.0%	230.0 / 8.2	75	L264L (CTG→TTG)	insK	IS150 conserved protein InsB
Reads supporting (aligned to +/- strand): ref base G (32/37); new base A (3/3); total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

AGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTA  >  W3110S.gb/3918366‑3918492

aGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAt                                                          >  1:2380111/1‑71 (MQ=255)

 gTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCCTTTAATTTCAGGCTAAt                                                          >  1:2066078/1‑70 (MQ=255)

  ttAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCt                                                             <  1:701612/66‑1 (MQ=255)

  ttAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCt                                                             <  1:1627996/66‑1 (MQ=255)

  ttAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAttc                                                        >  1:465302/1‑71 (MQ=255)

    aaaCACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAttctt                                                      >  1:2170543/1‑71 (MQ=255)

    aaaCACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAttct                                                       >  1:1908680/1‑70 (MQ=255)

       cacGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTctg                                                   >  1:103183/1‑71 (MQ=255)

       cacGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTctg                                                   <  1:1830010/71‑1 (MQ=255)

        acGTGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCt                                                             <  1:2057571/60‑1 (MQ=255)

             gCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttg                                             <  1:433004/71‑1 (MQ=255)

             gCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttg                                             >  1:2655751/1‑71 (MQ=255)

             gCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCtgttg                                             <  1:1066394/71‑1 (MQ=255)

               atatAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttg                                             >  1:2284313/1‑69 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTctgc                                                  <  1:152180/63‑1 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTctgc                                                  <  1:2712806/63‑1 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttgt                                            <  1:2596696/69‑1 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttgt                                            <  1:1232281/69‑1 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttg                                             <  1:1380366/68‑1 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtag                                          <  1:2126709/71‑1 (MQ=255)

                tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtag                                          <  1:2054155/71‑1 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAgg                                                               >  1:978622/1‑48 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:2154536/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:2718108/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:2721114/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:623283/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:861350/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:86737/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:2127802/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagta                                        >  1:1498507/1‑71 (MQ=255)

                  taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagt                                         >  1:1900538/1‑70 (MQ=255)

                      tCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAtt                                                         >  1:1585527/1‑50 (MQ=255)

                       cTGATTCCGATATTCAATTGGAGTCAGACC‑TTTAATTTCAGGCTaa                                                           >  1:739422/1‑46 (MQ=39)

                          aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAttc                                                        >  1:1784343/1‑47 (MQ=255)

                          aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAata                                 <  1:2534966/70‑1 (MQ=255)

                          aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAata                                 >  1:862024/1‑70 (MQ=255)

                          aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAata                                 <  1:1566231/70‑1 (MQ=255)

                          aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAata                                 <  1:1566233/70‑1 (MQ=255)

                          aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAat                                  <  1:947031/69‑1 (MQ=255)

                           ttCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagt                                         <  1:1688903/61‑1 (MQ=255)

                           ttCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAatata                               >  1:711964/1‑71 (MQ=255)

                           ttCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAatata                               <  1:2642923/71‑1 (MQ=255)

                           ttCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAatata                               <  1:2509868/71‑1 (MQ=255)

                            tCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgtagt                                         >  1:2618433/1‑60 (MQ=255)

                             ccGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTATTCTGCtgt                                               >  1:562974/1‑53 (MQ=255)

                              cGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTc                            <  1:2047013/71‑1 (MQ=255)

                                 tatTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGt                         <  1:2418834/71‑1 (MQ=255)

                                   ttCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTa                        <  1:157496/70‑1 (MQ=255)

                                     cAATTGGAGTCAGACCTTTTAATTTCAGGCTAAttctt                                                      >  1:1746349/1‑38 (MQ=255)

                                     cAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAatata                               >  1:2022840/1‑61 (MQ=255)

                                     cAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAatata                               >  1:729393/1‑61 (MQ=255)

                                     cAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTaa                       >  1:1963565/1‑69 (MQ=255)

                                      aaTTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCaa                                   <  1:2057320/56‑1 (MQ=255)

                                       aTTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCtgttgt                                            <  1:2611601/46‑1 (MQ=255)

                                       aTTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAatatat                              <  1:1251064/60‑1 (MQ=255)

                                        ttGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCa                  <  1:1585985/71‑1 (MQ=255)

                                         tGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCAt                 >  1:2457643/1‑71 (MQ=255)

                                             gTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCaa                                   <  1:2053384/49‑1 (MQ=255)

                                             gTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAatata                               <  1:2174640/53‑1 (MQ=255)

                                             gTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCtt             <  1:1089302/71‑1 (MQ=255)

                                                aGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg          >  1:1332718/1‑71 (MQ=255)

                                                aGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg          >  1:917216/1‑71 (MQ=255)

                                                aGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCAGTAACAGCATCCTTCAg          >  1:501935/1‑71 (MQ=255)

                                                  aCCTTTTAATTTCAGGCTAATTCTTCTGCTGTTgta                                           <  1:914407/36‑1 (MQ=255)

                                                  aCCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGtt        >  1:1786448/1‑71 (MQ=255)

                                                   ccTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTc       >  1:309400/1‑71 (MQ=255)

                                                    cTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCa                                    <  1:623192/41‑1 (MQ=255)

                                                    cTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTc            <  1:210511/65‑1 (MQ=255)

                                                     ttttAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCaa                                   <  1:2265841/41‑1 (MQ=255)

                                                       ttAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCt    <  1:1965679/70‑1 (MQ=255)

                                                         aaTTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta  <  1:1801866/70‑1 (MQ=255)

                                                         aaTTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta  <  1:1038664/70‑1 (MQ=255)

                                                          aTTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta  <  1:1035455/69‑1 (MQ=255)

                                                           tttCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCa                  <  1:1199023/52‑1 (MQ=255)

                                                           tttCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCa                  <  1:2296033/52‑1 (MQ=255)

                                                             tCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTaa                       >  1:1989489/1‑45 (MQ=255)

AGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTA  >  W3110S.gb/3918366‑3918492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A