Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
434,571 |
A→T |
100% |
W66R (TGG→AGG) |
fldA ← |
flavodoxin 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 434,571 | 0 | A | T | 100.0%
| 78.8
/ NA
| 22 | W66R (TGG→AGG) | fldA | flavodoxin 1 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/22); total (0/22) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAG > minE/434567‑434637
|
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:415190/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:88771/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:72200/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:644954/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:583217/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:55056/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:547956/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:484546/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:465028/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:458507/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:130090/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:412328/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:406834/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:346673/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:327817/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:311288/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:291534/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:270319/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:148471/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:14124/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGCTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:302612/71‑1 (MQ=255)
tCCCTGTCACACTGCGCTTCGCCGTAATACCACGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg < 1:87631/71‑1 (MQ=255)
|
TCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAG > minE/434567‑434637
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
