Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
816,173 |
A→G |
100% |
E82E (GAA→GAG) |
hemA → |
glutamyl tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 816,173 | 0 | A | G | 100.0%
| 130.3
/ NA
| 36 | E82E (GAA→GAG) | hemA | glutamyl tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/0); total (36/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCG > minE/816162‑816232
|
gttAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCAATTTAATGCg > 1:605551/2‑71 (MQ=255)
ctaaaTGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:488931/4‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAacga > 1:645477/1‑50 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGAcgc > 1:625169/1‑53 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:554495/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:372129/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:372910/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:404085/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:442904/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:4701/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:496147/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:525261/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:34031/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:601866/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:623748/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:636438/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:67919/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:87560/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:15785/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:112010/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:119727/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:126985/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:128848/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:139904/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:141715/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:142131/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:146501/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:107829/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:203604/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:22352/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:241315/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:2612/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:264964/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:296748/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:302676/1‑71 (MQ=255)
cTTAATGAAGAGGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg > 1:319923/1‑71 (MQ=255)
|
CTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCG > minE/816162‑816232
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
