Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
825,185 |
C→T |
100% |
E32K (GAG→AAG) |
ychN ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 825,185 | 0 | C | T | 100.0%
| 61.8
/ NA
| 18 | E32K (GAG→AAG) | ychN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/18); total (0/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTCTCCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTGCGC > minE/825181‑825250
|
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:359329/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:70830/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:651022/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:630877/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:550986/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:545064/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:475391/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:470030/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:455380/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:36599/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:175200/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:346113/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:338908/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:324790/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:313475/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:294383/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:253713/70‑1 (MQ=255)
ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:208587/70‑1 (MQ=255)
tcttctGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:281150/69‑1 (MQ=255)
|
CTCTCCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTGCGC > minE/825181‑825250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
