Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R2 68 0.0 1607493 93.4% 1501398 66.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,717,229 G→A intergenic (‑45/‑162) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,717,2290GA100.0% 475.6 / NA 142intergenic (‑45/‑162)yfiF/trxCpredicted methyltransferase/thioredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (142/0);  total (142/0)

Only 100 of 142 total aligned reads displayed.

CGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAT  >  W3110S.gb/2717207‑2717275
                      |                                              
cgAAGCGCGCTGATTACCCGAAATCCGCGCGGTTCGcc                                 >  1:1450076/1‑38 (MQ=38)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCg                        >  1:290061/1‑47 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAACGCCTGCGACTAt     >  1:1576108/1‑66 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAACGCCTGCGACTATCa   >  1:427075/1‑68 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAACGCCTGCGACTATCAt  >  1:256914/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCt             >  1:198947/1‑58 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACt       >  1:641352/1‑64 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt     >  1:555243/1‑66 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa   >  1:1402630/1‑68 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa   >  1:1333619/1‑68 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa   >  1:142386/1‑68 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa   >  1:1263844/1‑68 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa   >  1:736305/1‑68 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:517325/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:513620/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:54533/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:545483/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:468897/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:46400/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:460962/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:402834/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:383449/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:562534/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:378169/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:25681/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:564002/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:574492/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:624873/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:791292/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:973329/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:963626/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:936463/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:92393/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:92335/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:911988/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:910040/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:856558/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:837349/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:816604/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:800119/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:598780/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:790755/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:775599/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:767111/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:752926/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:740335/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:726074/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:704548/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:654492/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:625887/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:216003/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:619767/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1134462/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:243829/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:134482/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1331848/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1272265/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1229054/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1211859/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1207300/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1187833/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1186630/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1143159/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1135222/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1363818/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1132357/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1130684/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1098155/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1095799/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1067282/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1061659/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1047396/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1031244/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1030489/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1023480/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1017105/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1595364/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:221222/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1001873/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:200760/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:200548/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:200444/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:18310/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:175586/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:165687/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1365673/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1593074/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:158860/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1585825/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1560798/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1481735/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1446121/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1423180/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1414613/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1403574/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1379066/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:1377707/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCACCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:303385/1‑69 (MQ=255)
cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTGCGCCATGAtt                           >  1:315381/1‑44 (MQ=38)
cgAAGCGAGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt  >  1:81558/1‑69 (MQ=255)
                      |                                              
CGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAT  >  W3110S.gb/2717207‑2717275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A