Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R2 68 0.0 1607493 93.4% 1501398 66.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,974,186 T→C D450D (GAT→GAC)  gadA → glutamate decarboxylase A, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,974,1860TC100.0% 386.2 / NA 104D450D (GAT→GAC) gadAglutamate decarboxylase A, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (104/0);  total (104/0)

Only 100 of 104 total aligned reads displayed.

AGCGATCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTA  >  W3110S.gb/3974181‑3974226
     |                                        
aGCGACCGCCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:904274/1‑46 (MQ=11)
aGCGACCACCCGAAACTGCAGGGTATTGCCcagcag            >  1:120722/1‑36 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCttt   >  1:1368672/1‑45 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCttt   >  1:559658/1‑45 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:465892/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:630565/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:620273/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:611329/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:602970/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:587288/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:571797/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:549148/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:529492/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:477124/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:470143/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:651409/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:458947/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:448386/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:420151/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:420093/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:405636/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:40382/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:387589/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:367937/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:35427/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:345237/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:32312/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:830122/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:981550/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:977370/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:973202/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:971586/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:931424/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:925732/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:911702/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:883351/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:872004/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:855589/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:841791/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:641929/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:826399/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:823845/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:79137/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:77964/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:746938/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:736169/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:728539/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:710090/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:698454/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:687094/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:320888/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1199022/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:131225/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1298231/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1296793/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1284571/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1268529/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1268197/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1261874/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1251960/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1250812/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1236698/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1206570/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1321683/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:118867/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:117911/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:115511/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1143185/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:113931/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1124312/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1101351/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:110130/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1099/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1081295/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1050793/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1545717/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:26279/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:257855/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:223286/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:199775/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:186462/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:179379/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:165501/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:162395/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1590612/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1547584/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1546549/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1043989/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1522416/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1489148/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:148739/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1456781/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1396283/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1392995/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1390017/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1387347/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1377910/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1366707/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTa  >  1:1358873/1‑46 (MQ=255)
aGCGACCACCCGAAACTGCAGGGTATTGCCCAGAAGAACAGCTTTa  >  1:960736/1‑46 (MQ=11)
     |                                        
AGCGATCACCCGAAACTGCAGGGTATTGCCCAGCAGAACAGCTTTA  >  W3110S.gb/3974181‑3974226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A