Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I2 R2	36	87.2	4157695	97.3%	4045437	63.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	474,288	T→C	V617A (GTT→GCT)	mngA →	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	474,288	0	T	C	100.0%	133.0 / NA	37	V617A (GTT→GCT)	mngA	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/21); total (16/21)

GACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCC  >  minE/474229‑474353

gACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTAt                                                                <  1:3394259/63‑1 (MQ=255)

 acacCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAAt                                                       <  1:3544357/71‑1 (MQ=255)

     ccGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGc                                                   <  1:315567/71‑1 (MQ=255)

       ggTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTg                                                 >  1:4153884/1‑71 (MQ=255)

              gCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCaa                                                        >  1:2207051/1‑57 (MQ=255)

                 ttttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAAtt                                                      <  1:3448433/56‑1 (MQ=255)

                   ttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATggc                                                             >  1:160577/1‑47 (MQ=255)

                   ttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATggc                                                             >  1:3268994/1‑47 (MQ=255)

                   ttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCgg                                      <  1:2071584/70‑1 (MQ=255)

                    tctcGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCa                                    >  1:1149302/1‑71 (MQ=255)

                     ctcGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCa                                    <  1:775854/70‑1 (MQ=255)

                        gCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtgg                                <  1:3619957/71‑1 (MQ=255)

                        gCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtg                                 <  1:3132946/70‑1 (MQ=255)

                         ctctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggt                               <  1:3582368/71‑1 (MQ=255)

                         ctctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCAGCATtggt                               <  1:3389293/71‑1 (MQ=255)

                          tctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggt                               <  1:4077116/70‑1 (MQ=255)

                           ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGTCTGGTTTGGCGCgg                                      <  1:1546098/62‑1 (MQ=255)

                           ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGtt                                              <  1:3082258/54‑1 (MQ=255)

                           ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGtt                                              <  1:692376/54‑1 (MQ=255)

                           ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCgg                                      <  1:202254/62‑1 (MQ=255)

                              tttACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCt                                                  >  1:318858/1‑47 (MQ=255)

                              tttACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTggggg                          >  1:3344142/1‑71 (MQ=255)

                                tACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGc                         <  1:2433325/70‑1 (MQ=255)

                                   ttCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGttt                                             <  1:319274/47‑1 (MQ=255)

                                   ttCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggtg                              <  1:162138/62‑1 (MQ=255)

                                     cATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTgg                                           >  1:3455243/1‑47 (MQ=255)

                                     cATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAt                   >  1:3631371/1‑71 (MQ=255)

                                     cATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAt                   >  1:2571372/1‑71 (MQ=255)

                                       tGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAtct                 >  1:664297/1‑71 (MQ=255)

                                         aTAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAtctc                <  1:2812021/70‑1 (MQ=255)

                                          tAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGa              >  1:98055/1‑71 (MQ=255)

                                          tAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATAGGTGGGGGCTCCAATCTCg               >  1:1658639/1‑70 (MQ=255)

                                           aaTGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGAc             >  1:625031/1‑71 (MQ=255)

                                            aTGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACt            >  1:3358715/1‑71 (MQ=255)

                                                  cgggcggTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAAt       <  1:392371/70‑1 (MQ=255)

                                                   gggcggTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATtc     <  1:3591861/71‑1 (MQ=255)

                                                       ggTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTcc  >  1:3054695/1‑70 (MQ=255)

GACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCC  >  minE/474229‑474353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A