Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I2 R2 36 87.2 4157695 97.3% 4045437 63.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 786,414 G→A T173I (ACC→ATC)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE786,4140GA100.0% 287.3 / NA 91T173I (ACC→ATC) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/47);  total (44/47)

AACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCA  >  minE/786348‑786480
                                                                  |                                                                  
aaCTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 <  1:3595336/70‑1 (MQ=255)
aaCTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACg                                                                <  1:531145/71‑1 (MQ=255)
aaCTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACg                                                                <  1:773504/71‑1 (MQ=255)
  cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               >  1:1242422/1‑70 (MQ=255)
  cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               >  1:1288843/1‑70 (MQ=255)
  cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               >  1:3050164/1‑70 (MQ=255)
     ttCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGaa                                                            <  1:2346653/70‑1 (MQ=255)
      tCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 <  1:1871498/64‑1 (MQ=255)
      tCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGaa                                                            >  1:3575554/1‑69 (MQ=255)
       ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAagag                                                         >  1:1239491/1‑71 (MQ=255)
       ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAagag                                                         >  1:2513460/1‑71 (MQ=255)
       ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:1189288/70‑1 (MQ=255)
       ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:807891/70‑1 (MQ=255)
       ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:1926998/70‑1 (MQ=255)
        cAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:3817815/69‑1 (MQ=255)
        cAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAgg                                                        <  1:72353/71‑1 (MQ=255)
         aGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               <  1:3845638/63‑1 (MQ=255)
         aGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGc                                                       >  1:2101925/1‑71 (MQ=255)
         aGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGc                                                       >  1:236296/1‑71 (MQ=255)
          gAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATa                                                                  <  1:2889455/59‑1 (MQ=255)
             ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:2556033/71‑1 (MQ=255)
             ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   >  1:3947362/1‑71 (MQ=255)
             ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:434185/71‑1 (MQ=255)
             ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:714067/71‑1 (MQ=255)
              cACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGGCGGTGGCTTCGATACGGGAAGAGGCTAcgc                                                  <  1:2101926/71‑1 (MQ=255)
                  ttGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 <  1:2035373/52‑1 (MQ=255)
                  ttGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               >  1:1278187/1‑70 (MQ=255)
                  ttGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               >  1:3337690/1‑70 (MQ=255)
                     tAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGAt                                            <  1:305480/70‑1 (MQ=255)
                     tAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGAt                                            <  1:25472/70‑1 (MQ=255)
                     tAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           <  1:3128285/71‑1 (MQ=255)
                      aaTGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           >  1:2689002/1‑70 (MQ=255)
                       aTGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATaaa                                         <  1:474564/71‑1 (MQ=255)
                        tGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:456918/60‑1 (MQ=255)
                        tGCTTGTCGATATGACTATCGTCTTGCAGGGCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:1291612/60‑1 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 >  1:3470792/1‑44 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 >  1:3118402/1‑44 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTa                                                     >  1:857731/1‑56 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTa                                                     >  1:677005/1‑56 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcgcg                                                 >  1:3135284/1‑60 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcgcg                                                 >  1:1603622/1‑60 (MQ=255)
                          cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATAc                                      >  1:2454742/1‑71 (MQ=255)
                           ttGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATAc                                      >  1:1097853/1‑70 (MQ=255)
                            tGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCa                                    >  1:3476763/1‑71 (MQ=255)
                              tCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATa                                  <  1:2983647/71‑1 (MQ=255)
                               cGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGa                                             <  1:2857723/59‑1 (MQ=255)
                               cGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGa                                             <  1:696203/59‑1 (MQ=255)
                               cGATATGACTATCGTCTTGCAGGTCGGGGTCTTCGATACGGGAAGAGGCTACGCGGTGa                                             <  1:2251679/59‑1 (MQ=255)
                                gATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaa                                <  1:2274529/71‑1 (MQ=255)
                                gATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaa                                <  1:3730393/71‑1 (MQ=255)
                                gATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaa                                 >  1:4149382/1‑70 (MQ=255)
                                 atatGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaa                                >  1:336140/1‑70 (MQ=255)
                                  tatGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:1384677/54‑1 (MQ=255)
                                  tatGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:3074776/54‑1 (MQ=255)
                                    tGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATaaa                                         >  1:127187/1‑58 (MQ=255)
                                    tGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAAcc                            >  1:3514181/1‑71 (MQ=255)
                                      aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          <  1:224384/71‑1 (MQ=255)
                                      aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          >  1:4088675/1‑71 (MQ=255)
                                      aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          <  1:359310/71‑1 (MQ=255)
                                      aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          >  1:1916881/1‑71 (MQ=255)
                                       cTATCGTCTTGCAGGTCTGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGAc                         >  1:3954065/1‑71 (MQ=255)
                                       cTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGAc                         >  1:3980651/1‑71 (MQ=255)
                                        tATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACt                        >  1:632274/1‑71 (MQ=255)
                                          tCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaaaa                              >  1:1184295/1‑63 (MQ=255)
                                            gTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCa                     <  1:1142331/70‑1 (MQ=255)
                                             tCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           <  1:2898677/47‑1 (MQ=255)
                                               ttGCAGGTCGGTGTCTTCGATACGTGAAGAGGCTACGCGGt                                               <  1:2915888/41‑1 (MQ=255)
                                               ttGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGc                 >  1:223776/1‑71 (MQ=255)
                                               ttGCAGGGCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:80708/41‑1 (MQ=255)
                                                tGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           <  1:2490853/44‑1 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:1002008/39‑1 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATa                                       >  1:1735789/1‑47 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                <  1:3568300/70‑1 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                >  1:986188/1‑70 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                <  1:134808/70‑1 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                >  1:440517/1‑70 (MQ=255)
                                                 gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                <  1:2903953/70‑1 (MQ=255)
                                                  cAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTg               <  1:2524727/70‑1 (MQ=255)
                                                   aGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGAt             >  1:1275405/1‑71 (MQ=255)
                                                        ggTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATc            >  1:168602/1‑67 (MQ=255)
                                                         gtgtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcaccac       <  1:1928687/71‑1 (MQ=255)
                                                         gtgtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcaccac       >  1:3278582/1‑71 (MQ=255)
                                                         gtgtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcaccac       >  1:3700004/1‑71 (MQ=255)
                                                           gtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATAcc                                     >  1:381769/1‑39 (MQ=255)
                                                           gtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCg     >  1:2166703/1‑71 (MQ=255)
                                                            tCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcacc         <  1:2388235/66‑1 (MQ=255)
                                                            tCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGc    <  1:2455613/71‑1 (MQ=255)
                                                            tCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGc    >  1:1288117/1‑71 (MQ=255)
                                                              ttCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaaa                               <  1:3452491/42‑1 (MQ=255)
                                                              ttCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcacc         >  1:1669402/1‑64 (MQ=255)
                                                              ttCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCa  >  1:2533128/1‑71 (MQ=255)
                                                                  |                                                                  
AACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCA  >  minE/786348‑786480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A