Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
71 |
95.7 |
2922988 |
91.7% |
2680379 |
65.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
813,089 |
T→A |
100% |
intergenic (‑72/+53) |
ychM ← / ← prsA |
predicted transporter/phosphoribosylpyrophosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 813,089 | 0 | T | A | 100.0%
| 21.3
/ NA
| 8 | intergenic (‑72/+53) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (8/0); total (8/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCC > minE/813044‑813115
|
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGa > 1:1669998/1‑58 (MQ=255)
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:1405634/1‑71 (MQ=255)
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:2477258/1‑71 (MQ=255)
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:2582457/1‑71 (MQ=255)
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:2670660/1‑71 (MQ=255)
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:913709/1‑71 (MQ=255)
atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTAAAGACAAAAAAACccgc > 1:846911/1‑71 (MQ=255)
atcTGT‑AACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgcc > 1:1858073/1‑71 (MQ=255)
|
ATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCC > minE/813044‑813115
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
