Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,635,047 |
G→A |
31.4% |
A53V (GCG→GTG) |
yjjK ← |
fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,635,047 | 0 | G | A | 31.4%
| 69.1
/ 40.9
| 51 | A53V (GCG→GTG) | yjjK | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (19/16); new base A (10/6); total (29/22) |
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCC > W3110S.gb/4634982‑4635111
|
gCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:481141/70‑1 (MQ=255)
gCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:1849125/70‑1 (MQ=255)
aaCCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATgcg > 1:418372/1‑70 (MQ=255)
aaCCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATgcg > 1:3217051/1‑70 (MQ=255)
aCCAATCTTGATGTCTGGCTGCGGACGCTCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATgcg > 1:2746531/1‑69 (MQ=255)
aCCAATCTTGATGTATGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATgcg > 1:3320895/1‑69 (MQ=255)
cAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAAAGCCCACCATAATGCgcag > 1:2723673/1‑70 (MQ=255)
tCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATgcgc < 1:165707/65‑1 (MQ=255)
ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGt > 1:2456202/1‑71 (MQ=255)
ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGt > 1:2184264/1‑71 (MQ=255)
ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGt > 1:3245829/1‑71 (MQ=255)
gATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTg > 1:1395484/1‑70 (MQ=255)
aTGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTgg < 1:1373892/70‑1 (MQ=255)
gTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:2155851/51‑1 (MQ=255)
tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:1397429/1‑52 (MQ=255)
tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:2675922/1‑52 (MQ=255)
tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:2612925/1‑52 (MQ=255)
tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:659939/1‑50 (MQ=255)
tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:338909/1‑50 (MQ=255)
tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAc > 1:192945/1‑71 (MQ=255)
ggACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCgcgc < 1:2150416/70‑1 (MQ=255)
cTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGa < 1:1053689/71‑1 (MQ=255)
cTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAggg < 1:1925749/48‑1 (MQ=255)
aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAccc < 1:1957942/70‑1 (MQ=255)
aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAcc > 1:2434319/1‑69 (MQ=255)
aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAcc > 1:1153142/1‑69 (MQ=255)
ccTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg < 1:3958524/71‑1 (MQ=255)
ttCTATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:1111287/38‑1 (MQ=255)
ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:1005639/38‑1 (MQ=255)
ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:3761847/38‑1 (MQ=255)
ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:938520/38‑1 (MQ=255)
ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:3377509/38‑1 (MQ=255)
ttCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:50662/1‑71 (MQ=255)
tCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:393413/1‑70 (MQ=255)
tCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:3421855/1‑70 (MQ=255)
tCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCGAGGa > 1:3629999/1‑70 (MQ=255)
gTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:4015032/1‑63 (MQ=255)
gTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:2676673/1‑63 (MQ=255)
tCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCaa < 1:199268/70‑1 (MQ=255)
cTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCaa < 1:1085341/69‑1 (MQ=255)
tttATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAtt > 1:2846479/1‑70 (MQ=255)
tttATCAATGCCCACCATAATGCGCAGCAGGGTGGACt < 1:3132099/38‑1 (MQ=255)
tttATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTa > 1:4032293/1‑40 (MQ=255)
tATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAtt < 1:985355/68‑1 (MQ=255)
tATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCa > 1:2647606/1‑57 (MQ=255)
tATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:2241044/1‑58 (MQ=255)
cAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcc > 1:393508/1‑71 (MQ=255)
aaTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGccc > 1:3189360/1‑71 (MQ=255)
aTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc < 1:1666482/71‑1 (MQ=255)
aTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc < 1:1454469/71‑1 (MQ=255)
aTGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc < 1:810722/71‑1 (MQ=255)
|
GCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCC > W3110S.gb/4634982‑4635111
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
