Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
442,546 |
T→C |
73.3% |
N406D (AAT→GAT) |
speF ← |
ornithine decarboxylase isozyme, inducible |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 442,546 | 0 | T | C | 73.3%
| 66.0
/ 20.6
| 60 | N406D (AAT→GAT) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base T (4/12); new base C (14/29); total (19/41) |
| Fisher's exact test for biased strand distribution p-value = 7.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
CCATTTACCACACAATCCATCCACATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCGTTGTTC > minE/442479‑442607
|
ccATTTACCACACAATCCATCCACATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATTc > 1:1538987/1‑69 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTTATATCCAGTGCGGCAAACAGCgg < 1:156933/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1749499/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:2625014/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:2613241/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:2764650/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:2165857/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:216416/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:340393/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:3431484/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1969440/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:2776968/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:485245/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1888121/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:2942192/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1827818/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:732016/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:3121905/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1098148/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:11172/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1309735/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1351515/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1743064/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:641158/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:619917/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1573029/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:16623/69‑1 (MQ=255)
catccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1723208/69‑1 (MQ=255)
atccaCATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:351809/68‑1 (MQ=255)
tACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACgcgc > 1:2596837/1‑69 (MQ=255)
gCTGACACCTTCATGCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCgg < 1:1465142/51‑1 (MQ=255)
acCTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTg > 1:3152856/1‑69 (MQ=255)
cTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGca < 1:298715/69‑1 (MQ=255)
cTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGca < 1:1839306/69‑1 (MQ=255)
cTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGca < 1:3306991/69‑1 (MQ=255)
cTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGca < 1:424695/69‑1 (MQ=255)
cTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGca < 1:748965/69‑1 (MQ=255)
cTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGca < 1:1113668/69‑1 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTg > 1:764278/1‑61 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:37383/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:1116127/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:74988/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:1387815/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:1517652/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:541367/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:1929268/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:1929354/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:1938241/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:3407937/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:3389568/1‑69 (MQ=255)
gCATTTTGGCGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:2322489/1‑69 (MQ=255)
gCATTTTGGCGTTGATATACAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:2295372/1‑69 (MQ=255)
gCATTTTGGAGTTGATATCCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATaa > 1:145196/1‑69 (MQ=255)
cGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCgttg < 1:2030221/68‑1 (MQ=255)
cGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCgttg < 1:531703/68‑1 (MQ=255)
cGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCgttg < 1:1521941/68‑1 (MQ=255)
cGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCgttg < 1:3336962/68‑1 (MQ=255)
gTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCgttgtt < 1:1989352/69‑1 (MQ=255)
gTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCgttgtt < 1:3389806/69‑1 (MQ=255)
ttGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCGTTGTTc > 1:3109899/1‑69 (MQ=255)
|
CCATTTACCACACAATCCATCCACATATTACGACCGCTGACACCTTCATGCATTTTGGCGTTGATATTCAGTGCGGCAAACAGCGGATAGAACGGGCTGGTGGAGGCGTGCATCATAAAGGCGTTGTTC > minE/442479‑442607
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
