Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
443,246 |
G→A |
22.6% |
H172H (CAC→CAT) |
speF ← |
ornithine decarboxylase isozyme, inducible |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 443,246 | 0 | G | A | 22.6%
| 89.5
/ 17.8
| 66 | H172H (CAC→CAT) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base G (16/35); new base A (7/8); total (23/43) |
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 |
TTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCA > minE/443188‑443309
|
ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:3270332/69‑1 (MQ=255)
ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:993111/69‑1 (MQ=255)
ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:2003341/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGTATcagcag < 1:2117492/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:1027132/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:3093638/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:2895602/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:2691573/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:629468/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:1373015/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:112846/69‑1 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca > 1:221263/1‑68 (MQ=255)
tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:2345477/1‑64 (MQ=255)
ttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:1429592/1‑63 (MQ=255)
ttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:2794263/1‑63 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:1188877/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:951395/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:732949/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:2589691/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:1468049/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:3334457/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:3036427/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGAAGATCGcc < 1:1335858/69‑1 (MQ=255)
ggCATTAAACACTTTTGCCGCATGTAGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:844562/69‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2229102/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2517627/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1458228/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2981123/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1461687/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2089219/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1841596/68‑1 (MQ=255)
cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1765438/68‑1 (MQ=255)
aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:360799/1‑69 (MQ=255)
aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:2932650/1‑69 (MQ=255)
aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:787286/1‑69 (MQ=255)
aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:1457489/1‑69 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATcagaa > 1:1982993/1‑53 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:2524431/1‑68 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:3301183/1‑68 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:352689/1‑68 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:2602378/1‑68 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:1189236/1‑68 (MQ=255)
aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:1318859/1‑68 (MQ=255)
cacTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTAc < 1:2519555/69‑1 (MQ=255)
cTTTTGCCGCATGTTGCTGTGCAATGCATGTCGCGCCTTCGTGAATc > 1:1257114/1‑47 (MQ=255)
cTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGt > 1:2940839/1‑69 (MQ=255)
cTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGt > 1:2286845/1‑69 (MQ=255)
ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:3417022/1‑69 (MQ=255)
ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:1107246/1‑69 (MQ=255)
ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:3161415/1‑69 (MQ=255)
ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:2963793/1‑69 (MQ=255)
ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:317494/1‑69 (MQ=255)
gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:361372/69‑1 (MQ=255)
gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:916189/69‑1 (MQ=255)
gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:1830118/69‑1 (MQ=255)
gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:1852498/69‑1 (MQ=255)
gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:581962/69‑1 (MQ=255)
gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:3301474/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:2558143/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:2669583/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:2874992/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:1768381/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:1764390/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:3056255/69‑1 (MQ=255)
ccTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:1122716/69‑1 (MQ=255)
cTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCa < 1:1183350/68‑1 (MQ=255)
|
TTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCA > minE/443188‑443309
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
