Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
452,550 |
G→A |
34.3% |
R172H (CGC→CAC) |
phr → |
deoxyribodipyrimidine photolyase, FAD‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 452,550 | 0 | G | A | 34.3%
| 46.8
/ 33.3
| 64 | R172H (CGC→CAC) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base A (15/7); total (37/27) |
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAG > minE/452483‑452616
|
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1129515/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1145737/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:945079/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:701079/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1204091/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:415312/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:2187536/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:2178370/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1928257/1‑68 (MQ=255)
tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGACGCCGGAGTGCGTCGCTgcg > 1:998948/1‑68 (MQ=255)
tttACGCCTTTTAAGAATGCCTGGCTGAAACGGGTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:2938102/1‑68 (MQ=255)
tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:1123235/1‑68 (MQ=255)
tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:490187/1‑68 (MQ=255)
tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:445826/1‑68 (MQ=255)
tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:337611/1‑68 (MQ=255)
tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:134946/1‑68 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAg > 1:2717854/1‑68 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:181935/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:2603392/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:2605418/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:265014/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:447106/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:857962/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:2808036/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:850829/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:524756/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:3177408/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:1557723/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:985546/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:468708/1‑69 (MQ=255)
gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:489261/1‑69 (MQ=255)
gCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGa < 1:2918476/69‑1 (MQ=255)
tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:60730/68‑1 (MQ=255)
tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:329972/68‑1 (MQ=255)
tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:608943/68‑1 (MQ=255)
tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:215564/68‑1 (MQ=255)
tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:2194774/68‑1 (MQ=255)
tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:881025/68‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:1303224/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:94977/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:1185179/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:1171606/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:2296814/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:24416/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:2671803/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:415627/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:2560149/69‑1 (MQ=255)
ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGAGGATCGa < 1:2097339/69‑1 (MQ=255)
gAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATaga < 1:870012/69‑1 (MQ=255)
gAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATaga < 1:1468952/69‑1 (MQ=255)
gAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATaga < 1:2519815/69‑1 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:521613/1‑66 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:612876/1‑66 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2737384/1‑66 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2660126/1‑66 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2542580/1‑66 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2519245/1‑66 (MQ=255)
gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2031065/1‑66 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:798798/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:1023626/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:171342/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:316003/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2482986/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2247162/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2243116/1‑69 (MQ=255)
ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2131850/1‑69 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGATCCCGCGCCATCCATTACGCTGAATTATc < 1:347568/69‑1 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:1356270/69‑1 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:3322340/69‑1 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:3016770/69‑1 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:861349/69‑1 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:2411340/69‑1 (MQ=255)
gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:1967639/69‑1 (MQ=255)
cGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATcc > 1:2829707/1‑69 (MQ=255)
gcgcCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAg < 1:1255017/69‑1 (MQ=255)
gcgcCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAg < 1:3282142/69‑1 (MQ=255)
gcgcCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAg < 1:1499769/69‑1 (MQ=255)
|
TCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAG > minE/452483‑452616
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
