Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
454,239 |
G→T |
54.7% |
A282A (GCC→GCA) |
ybgH ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 454,239 | 0 | G | T | 54.7%
| ‑1.2
/ 45.3
| 42 | A282A (GCC→GCA) | ybgH | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (10/9); new base T (20/3); total (30/12) |
| Fisher's exact test for biased strand distribution p-value = 1.95e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 |
GCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTG > minE/454182‑454298
|
gcgGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGaacaa < 1:1440498/69‑1 (MQ=255)
gcgGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGaacaa < 1:3268128/69‑1 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCTCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:3045005/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:281568/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:913099/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:747532/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:707075/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:670355/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:645792/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:522320/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:488157/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:3027596/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:1135104/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:2783203/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:2656722/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:2622523/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:1648013/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:11688/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:129256/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:1534273/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:1692809/1‑69 (MQ=255)
gATATAAAGGCTAATCGAGATACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAg > 1:3081202/1‑69 (MQ=255)
aaGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTcac > 1:2105492/1‑69 (MQ=255)
aaGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTcac > 1:720240/1‑69 (MQ=255)
aaGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTcac > 1:265833/1‑69 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTg > 1:2933455/1‑52 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTg > 1:1965174/1‑52 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTg > 1:825206/1‑52 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTg > 1:32057/1‑52 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTg > 1:2335684/1‑52 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGc < 1:182124/67‑1 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGc < 1:1222627/67‑1 (MQ=255)
cGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGc < 1:1414855/67‑1 (MQ=255)
gCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCa < 1:286840/68‑1 (MQ=255)
gCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCa < 1:3367426/68‑1 (MQ=255)
gCTACCCCCCTGTTGTGCGAATGCCCAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCa < 1:3187811/68‑1 (MQ=255)
tACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCt < 1:1993122/69‑1 (MQ=255)
tACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCt < 1:1251521/69‑1 (MQ=255)
tACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCt < 1:1581935/69‑1 (MQ=255)
tACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCt < 1:811201/69‑1 (MQ=255)
ttgtGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTg > 1:58892/1‑69 (MQ=255)
ttgtGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTg > 1:1747834/1‑69 (MQ=255)
|
GCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTG > minE/454182‑454298
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
