Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
454,293 |
C→A |
64.8% |
R264R (CGG→CGT) |
ybgH ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 454,293 | 0 | C | A | 64.8%
| 62.7
/ 75.5
| 91 | R264R (CGG→CGT) | ybgH | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (21/11); new base A (35/24); total (56/35) |
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTC > minE/454230‑454356
|
ttgtGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTg > 1:58892/1‑69 (MQ=255)
ttgtGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTg > 1:1747834/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGtttt > 1:2305692/1‑68 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGtttt > 1:1790977/1‑68 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:11234/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:2110715/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:2266002/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:2382367/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:2927921/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:1940833/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:1872910/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:311570/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:395850/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:1662344/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:969270/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:772244/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:1169824/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTc > 1:911619/1‑69 (MQ=255)
ccAGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTAAGCTGTTTTTGGtttt > 1:633953/1‑68 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGtt > 1:321731/1‑64 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:971492/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:3383827/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2116057/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2211205/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2632299/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2652927/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:89835/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2989567/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:3020108/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:3140766/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:354016/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:1947926/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2022828/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:1724399/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:1511849/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:2050359/1‑69 (MQ=255)
aGAACAACATGCTGAAGAAGGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTg > 1:166451/1‑69 (MQ=255)
acaacaTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCtt < 1:277675/69‑1 (MQ=255)
acaacaTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCtt < 1:1179325/69‑1 (MQ=255)
cTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGAGATaa > 1:574652/1‑69 (MQ=255)
gaagaaGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGGTTCTGGTTTTCTGCTTTGCGATAAAt > 1:2918933/1‑69 (MQ=255)
aagGTGAGGGTAATAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:903632/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTAGGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:3430357/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:1658804/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:599329/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:1559521/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:795652/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:3288789/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:3252072/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:176228/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:1771575/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:1771753/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:1798430/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2088730/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:934281/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:1078636/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2954967/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2815624/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2778205/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2753931/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2736166/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2128877/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2697907/69‑1 (MQ=255)
aagGTGAGGGTAACAATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2526161/69‑1 (MQ=255)
ggTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAAttttt > 1:2545399/1‑67 (MQ=255)
gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:2870460/1‑69 (MQ=255)
gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:848124/1‑69 (MQ=255)
gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:665121/1‑69 (MQ=255)
gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:1966800/1‑69 (MQ=255)
gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:1725012/1‑69 (MQ=255)
gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:1166749/1‑69 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCGTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCa > 1:920826/1‑67 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:213550/1‑68 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:2674125/1‑68 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:2142653/1‑68 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:240434/1‑68 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:2345067/1‑68 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:128802/1‑68 (MQ=255)
aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAg > 1:2210798/1‑68 (MQ=255)
gtaacaATCAGCCCCAGCTCCTTACGCTGTTTTTGGTTTTCTGCTTTGCGATAAAttttt < 1:2029195/57‑1 (MQ=255)
gTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTaca < 1:2916414/69‑1 (MQ=255)
aaTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAg < 1:508749/69‑1 (MQ=255)
aaTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAg < 1:2671097/69‑1 (MQ=255)
aaTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAg < 1:2708345/69‑1 (MQ=255)
aaTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAg < 1:2712241/69‑1 (MQ=255)
ccccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaat < 1:208672/69‑1 (MQ=255)
ccccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaat < 1:2049142/69‑1 (MQ=255)
caccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaat < 1:2121961/67‑1 (MQ=255)
ccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaataa < 1:1749483/69‑1 (MQ=255)
tCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTc > 1:749914/1‑69 (MQ=255)
tCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTc > 1:709734/1‑69 (MQ=255)
|
TTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTC > minE/454230‑454356
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
